|
| Status |
Public on Aug 25, 2023 |
| Title |
20% MR, animal 67759 |
| Sample type |
RNA |
| |
|
| Source name |
granulosa cells, 20% MR, isolated after slaughter
|
| Organism |
Bos taurus |
| Characteristics |
age at slaughter: 235 d
follicle count: 83
rumen fistula: fistula
|
| Treatment protocol |
In the 10% group, the calves were fed with a maximum of 10% milk replacer/kg bodyweight. In contrast, the calves of the 20% group received a maximum of 20% milk replacer/kg bodyweight (BERGIN Milch LC 50) per day from an automatic calf feeder (Kälbermama Lifestart, Urban GmbH & Co. KG, Hude/Wüsting, Germany). The milk allowance was adjusted weekly after weighing.
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| Growth protocol |
The 10% group received the whole milk replacer allowance for ten weeks followed by a two-week transition period from milk feeding to sole solid feeding. During the transition period, the daily milk allowance was gradually reduced. For the 20% group the transition period started already in week eight but lasted for four weeks. For both experimental groups milk feeding stopped at week 12. During the first 12 weeks of life, the calves of both groups were able to consume hay and calf starter ad libitum. Hay ad libitum feeding was continued until week 14. The calf starter was limited to 2 kg/day from week 12 to 14 and thereafter gradually reduced until week 16. From week ten onwards, all calves additionally received a total-mixed ration ad libitum. After week 16, the total-mixed ration was the sole feed offered for ad libitum intake in both groups until sampling.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the RNeasy Kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated RNA were prepared according to the standard Affymetrix protocol (GeneChip® Expression 3’Amplification One-Cycle Target Labeling and Control Reagents).
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| |
|
| Hybridization protocol |
The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
|
| Scan protocol |
The scanning of the microarray was done with the GeneChip Scanner 3000 HR (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
|
| Data processing |
Raw data were processed with the Transcriptome Analysis Console 4.0 (V4.0.2; Affymetrix), where normalization, background reduction and a gene-level summary was done using the RMA method (Robust Multichip Average).
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| |
|
| Submission date |
Oct 31, 2022 |
| Last update date |
Aug 25, 2023 |
| Contact name |
Anja Baufeld |
| E-mail(s) |
baufeld@fbn-dummerstorf.de
|
| Organization name |
Research Institute for Farm Animal Biology
|
| Street address |
Wilhelm-Stahl-Allee 2
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16500 |
| Series (1) |
| GSE216927 |
Early milk-feeding regimes in calves exert long-term influence on the development of ovarian granulosa cells |
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