|
| Status |
Public on Nov 08, 2022 |
| Title |
Control_singlecell_rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Retina
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
condition: Control
cell type: retinal cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Papain dissociation to prepare single cell suspension or 0.01% Nonidet P40 lysis buffer to prepare single nuclei suspension
scRNA-Seq following 10x ver3.1 guideline
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
scRNA-Seq: Sequencing results were processed through the Cell Ranger pipeline (10x Genomics) with default parameters. Seurat V3 was used to perform downstream analysis following the standard pipeline.
Assembly: OryCun2.0
|
| |
|
| Submission date |
Nov 04, 2022 |
| Last update date |
Nov 08, 2022 |
| Contact name |
Clayton Pio Santiago |
| E-mail(s) |
csanti10@jhmi.edu
|
| Organization name |
Johns Hopkins University
|
| Department |
Neuroscience
|
| Street address |
733 North Broadway
|
| City |
Baltimore |
| State/province |
Maryland |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL26786 |
| Series (1) |
| GSE217333 |
Comparative analysis of single-cell and single-nucleus RNA-sequencing in a rabbit model of retinal detachment-related proliferative vitreoretinopathy |
|
| Relations |
| BioSample |
SAMN31611924 |
| SRA |
SRX18175233 |
