|
| Status |
Public on Apr 30, 2012 |
| Title |
mT4 |
| Sample type |
RNA |
| |
|
| Source name |
JoMa1-MYCN-induced tumor
|
| Organism |
Mus musculus |
| Characteristics |
cell line: derived from JoMa1-MYCN-induced tumor
|
| Growth protocol |
JoMa1 cells were grown as described (Maurer et al. Differentiation, 2007 (PMID 17381545)).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were harvested when they reached 80% confluency, and lysed using Trizol. RNA was purified using the QIAGEN RNeasy Mini Kit.
|
| Label |
biotin
|
| Label protocol |
Biotin labelling was performed according to the manufacturer's recommendations (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization to Mouse 430 2.0 arrays was performed according to the manufacturer's recommendations (Affymetrix).
|
| Scan protocol |
Scanning of chips was performed using a GeneArray scanner (Agilent).
|
| Description |
JoMa1 cells transfected with MYCN (JoMa1-MYCN) are tumorigenic in immunocompromised mice. Expression profiles of four independent tumors induced by JoMa1-MYCN were obtained.
|
| Data processing |
Data were RMA normalized using Genomics Suite 6.5 (Partek).
|
| |
|
| Submission date |
Feb 08, 2011 |
| Last update date |
Apr 30, 2012 |
| Contact name |
Alexander Schramm |
| E-mail(s) |
alexander.schramm@uk-essen.de
|
| Organization name |
University Hospital Essen
|
| Department |
Childrens Hospital
|
| Lab |
Hematology/Oncology Lab
|
| Street address |
Hufelandstr. 55
|
| City |
Essen |
| ZIP/Postal code |
45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27159 |
Expression profiling of the murine neural crest precursor cell line, JoMa1 |
|
