|
| Status |
Public on Dec 31, 2015 |
| Title |
HK-2 cells, Nnm, hd, t24h, biological rep 2, Tox1 |
| Sample type |
RNA |
| |
|
| Source name |
Human renal proximal tubular epithelial cells, treated with high dose Nnm, t24h
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HK-2
cell type: immortalized human renal proximal tubular epithelial cells
treatment: Nnm
treatment dosage: high
treatment duration: 24 hrs
|
| Treatment protocol |
Cells were seeded 24 hours before treatment on Greiner 6-well plates at a density of 7x10^5 cells per well with 2mls of medium per well. Cells were treated at ~90% confluency with the required doses for the required timepoints. Control cells were exposed to an appropriate concentration of the control vehicle.
|
| Growth protocol |
HK-2 cells were maintained in a 1-1 mixture of DMEM and Ham'sF12 containing 5 mM glucose supplemented with 10 ng/ml human recombinant EGF, 36 ng/ml hydrocortisone, 5 µg/ml bovine insulin, 5 µg/ml human transferrin, 5 ng/ml sodium selenite, 2 mM Glutamax (Gibco), 100 U/ml penicillin and 100 µg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy mini kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
15 ug fragmented labelled cRNA was hybridized for 16 hours at 45oC to Affymetrix HGU133_Plus 2.0 GeneChips, which were then stained and washed on an Affymetrix Fluidics Station 400.
|
| Scan protocol |
Fluorescence was read with an Affymetrix 3000 Scanner.
|
| Description |
Nnm-hd-t24h-rep-2
|
| Data processing |
Data was normalized using the gcrma library (version 2.8.1) of Bioconductor.
|
| |
|
| Submission date |
Feb 09, 2011 |
| Last update date |
Dec 31, 2015 |
| Contact name |
Peadar O'Gaora |
| Organization name |
UCD
|
| Street address |
Belfield
|
| City |
Dublin |
| ZIP/Postal code |
Dublin 4 |
| Country |
Ireland |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE27202 |
Expression data in HK-2 cells following exposure to Tox 1 compounds: CsA, Fk, Nnm and Ra |
| GSE27211 |
Gene expression changes in HK-2 cells following exposure to nephrotoxic compounds |
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