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Status |
Public on Dec 31, 2015 |
Title |
HK-2 cells, Fk and Ra, t12h, control sample, biological rep 3, Tox1 |
Sample type |
RNA |
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Source name |
Human renal proximal tubular epithelial cells, control for Fk and Ra, t12h
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Organism |
Homo sapiens |
Characteristics |
cell line: HK-2 cell type: immortalized human renal proximal tubular epithelial cells treatment: control treatment duration: 12 hrs
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Treatment protocol |
Cells were seeded 24 hours before treatment on Greiner 6-well plates at a density of 7x10^5 cells per well with 2mls of medium per well. Cells were treated at ~90% confluency with the required doses for the required timepoints. Control cells were exposed to an appropriate concentration of the control vehicle.
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Growth protocol |
HK-2 cells were maintained in a 1-1 mixture of DMEM and Ham'sF12 containing 5 mM glucose supplemented with 10 ng/ml human recombinant EGF, 36 ng/ml hydrocortisone, 5 µg/ml bovine insulin, 5 µg/ml human transferrin, 5 ng/ml sodium selenite, 2 mM Glutamax (Gibco), 100 U/ml penicillin and 100 µg/ml streptomycin.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the Qiagen RNeasy mini kit.
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Label |
biotin
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Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
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Hybridization protocol |
15 ug fragmented labelled cRNA was hybridized for 16 hours at 45oC to Affymetrix HGU133_Plus 2.0 GeneChips, which were then stained and washed on an Affymetrix Fluidics Station 400.
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Scan protocol |
Fluorescence was read with an Affymetrix 3000 Scanner.
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Description |
Fk_and_Ra-t12h_ctrl-rep-3
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Data processing |
Data was normalized using the gcrma library (version 2.8.1) of Bioconductor.
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Submission date |
Feb 09, 2011 |
Last update date |
Dec 31, 2015 |
Contact name |
Peadar O'Gaora |
Organization name |
UCD
|
Street address |
Belfield
|
City |
Dublin |
ZIP/Postal code |
Dublin 4 |
Country |
Ireland |
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Platform ID |
GPL570 |
Series (2) |
GSE27202 |
Expression data in HK-2 cells following exposure to Tox 1 compounds: CsA, Fk, Nnm and Ra |
GSE27211 |
Gene expression changes in HK-2 cells following exposure to nephrotoxic compounds |
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