|
| Status |
Public on Dec 31, 2022 |
| Title |
ExtracellularVesicles_model1 |
| Sample type |
RNA |
| |
|
| Source name |
Serum small extracellular vesicles
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: test
strain: Wister
gender: male
age: 12 weeks
tissue: serum small extracellular vesicles
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs in small extracellular vesicles were purified by Total Exosome RNA & Protein Isolation Kit (Cat.4478545, Invitrogen).
|
| Label |
biotin
|
| Label protocol |
We followed protocol of manual that is FlashTag(tm) Biotin HSR RNA Labeling Kits (Thermo Fisher Scientific, Inc., cat.# 901911) User Manual.
|
| |
|
| Hybridization protocol |
We followed protocol of manual that is Hybridization Control Kit (Thermo Fisher Scientific, Inc., cat.#900454) and Hybridization, Wash, and Stain Kit (Thermo Fisher Scientific, Inc.,cat.# 900720) User Manual.
|
| Scan protocol |
We followed manufacturer's protocol, and used GeneChip® Scanner 3000 7G.
|
| Data processing |
Data acquisition and normalization (RMA+DABG) were used Affymetrix® Expression Console™ Software.
|
| |
|
| Submission date |
Nov 08, 2022 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Shintaro Mandai |
| Organization name |
Tokyo Medical and Dental University, Graduate School of Medical and Dental Sciences
|
| Street address |
1-5-45 Yushima
|
| City |
Bunkyo |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8519 |
| Country |
Japan |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE217558 |
Signature analyses for circulating extracellular vesicle in chronic kidney disease |
|
