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Sample GSM672291 Query DataSets for GSM672291
Status Public on Dec 31, 2015
Title HK-2 cells, Kbr, hd, t24h, biological rep 3, All
Sample type RNA
 
Source name Human renal proximal tubular epithelial cells, treated with high dose Kbr, t24h
Organism Homo sapiens
Characteristics cell line: HK-2
cell type: immortalized human renal proximal tubular epithelial cells
treatment: Kbr
treatment dosage: high
treatment duration: 24 hrs
Treatment protocol Cells were seeded 24 hours before treatment on Greiner 6-well plates at a density of 7x10^5 cells per well with 2mls of medium per well. Cells were treated at ~90% confluency with the required doses for the required timepoints. Control cells were exposed to an appropriate concentration of the control vehicle.
Growth protocol HK-2 cells were maintained in a 1-1 mixture of DMEM and Ham'sF12 containing 5 mM glucose supplemented with 10 ng/ml human recombinant EGF, 36 ng/ml hydrocortisone, 5 µg/ml bovine insulin, 5 µg/ml human transferrin, 5 ng/ml sodium selenite, 2 mM Glutamax (Gibco), 100 U/ml penicillin and 100 µg/ml streptomycin.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the Qiagen RNeasy mini kit.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol 15 ug fragmented labelled cRNA was hybridized for 16 hours at 45oC to Affymetrix HGU133_Plus 2.0 GeneChips, which were then stained and washed on an Affymetrix Fluidics Station 400.
Scan protocol Fluorescence was read with an Affymetrix 3000 Scanner.
Description Kbr-hd-t24h-rep-3
Data processing Data was normalized using the gcrma library (version 2.8.1) of Bioconductor.
 
Submission date Feb 09, 2011
Last update date Dec 31, 2015
Contact name Peadar O'Gaora
Organization name UCD
Street address Belfield
City Dublin
ZIP/Postal code Dublin 4
Country Ireland
 
Platform ID GPL570
Series (2)
GSE27210 Expression data in HK-2 cells following exposure to all conditions
GSE27211 Gene expression changes in HK-2 cells following exposure to nephrotoxic compounds

Data table header descriptions
ID_REF
VALUE Log2 gcrma signal

Data table
ID_REF VALUE
1007_s_at 8.517977342
1053_at 9.440257591
117_at 3.088463639
121_at 10.75840724
1255_g_at 2.196038757
1294_at 2.950266694
1316_at 2.850643723
1320_at 2.999951236
1405_i_at 3.129056674
1431_at 2.700443118
1438_at 2.020013877
1487_at 7.01024137
1494_f_at 2.198728965
1552256_a_at 9.072312393
1552257_a_at 8.985577037
1552258_at 2.508157779
1552261_at 2.822438002
1552263_at 5.071231639
1552264_a_at 9.488341794
1552266_at 3.123333459

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM672291_ba_c_24h_hd_kbr_nc09.cel.gz 4.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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