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Status |
Public on Sep 08, 2011 |
Title |
gavin_OSCA_23_Canine_2 |
Sample type |
RNA |
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Source name |
primary osteosarcoma cell line
|
Organism |
Canis lupus familiaris |
Characteristics |
cell line: primary osteosarcoma breed: Golden Retriever Sex: MC age at diagnosis: 8.88 treatment: SOC survival: 13.97
|
Growth protocol |
Primary tumor cells or cryopreserved cultured cells. Snap-frozen primary tissue used for 6 samples.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was prepared using the RNeasy Mini Kit and QIAshredder column (Qiagen, Valencia, CA).
|
Label |
Biotin
|
Label protocol |
Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
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|
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Hybridization protocol |
Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
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Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
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Data processing |
CEL files were normalized using the GC-RMA algorithm as implemented in the Genedata Analyst Software Package.
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Submission date |
Feb 10, 2011 |
Last update date |
Sep 08, 2011 |
Contact name |
Aaron Sarver |
Organization name |
University of Minnesota
|
Department |
Masonic Cancer Center
|
Lab |
Biostatistics and Informatics
|
Street address |
425 East River Road
|
City |
Minneapolis |
State/province |
MN |
ZIP/Postal code |
55455 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE27217 |
Canine osteosarcoma cell lines and tissues |
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