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Sample GSM672507 Query DataSets for GSM672507
Status Public on Sep 08, 2011
Title gavin_OSCA_23_Canine_2
Sample type RNA
 
Source name primary osteosarcoma cell line
Organism Canis lupus familiaris
Characteristics cell line: primary osteosarcoma
breed: Golden Retriever
Sex: MC
age at diagnosis: 8.88
treatment: SOC
survival: 13.97
Growth protocol Primary tumor cells or cryopreserved cultured cells. Snap-frozen primary tissue used for 6 samples.
Extracted molecule total RNA
Extraction protocol RNA was prepared using the RNeasy Mini Kit and QIAshredder column (Qiagen, Valencia, CA).
Label Biotin
Label protocol Biotinylated cRNA were prepared according to standard Affymetrix protocol from 2.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 20 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine 2 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Data processing CEL files were normalized using the GC-RMA algorithm as implemented in the Genedata Analyst Software Package.
 
Submission date Feb 10, 2011
Last update date Sep 08, 2011
Contact name Aaron Sarver
Organization name University of Minnesota
Department Masonic Cancer Center
Lab Biostatistics and Informatics
Street address 425 East River Road
City Minneapolis
State/province MN
ZIP/Postal code 55455
Country USA
 
Platform ID GPL3738
Series (1)
GSE27217 Canine osteosarcoma cell lines and tissues

Data table header descriptions
ID_REF
VALUE GC-RMA signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 226.14212
AFFX-BioB-M_at 420.86163
AFFX-BioB-3_at 161.24796
AFFX-BioC-5_at 545.2401
AFFX-BioC-3_at 970.5501
AFFX-BioDn-5_at 2416.6045
AFFX-BioDn-3_at 2227.6096
AFFX-CreX-5_at 12426.286
AFFX-CreX-3_at 12577.237
AFFX-DapX-5_at 3.4018216
AFFX-DapX-M_at 2.9023814
AFFX-DapX-3_at 4.239029
AFFX-LysX-5_at 3.565684
AFFX-LysX-M_at 2.8066483
AFFX-LysX-3_at 3.8298197
AFFX-PheX-5_at 3.4832945
AFFX-PheX-M_at 2.9869435
AFFX-PheX-3_at 7.280215
AFFX-ThrX-5_at 5.0260825
AFFX-ThrX-M_at 3.8855534

Total number of rows: 43035

Table truncated, full table size 1260 Kbytes.




Supplementary file Size Download File type/resource
GSM672507.CEL.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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