|
| Status |
Public on Apr 07, 2023 |
| Title |
Human Pancreatic Islet scRNA 2 |
| Sample type |
SRA |
| |
|
| Source name |
Pancreatic Islet
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Pancreatic Islet
condition: Non-diabetic Normal
|
| Growth protocol |
serum free culture medium in non-adhesive culture plate in humidified atmosphere with 5% CO2 at 37C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
scRNA was filtered with Miltenyi Biotec’s dead cell removal kit
snRNA and graft snRNA extraction was done by Minute single nucleus isolation kit (cat#SN-047)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
integrated matrix
|
| Data processing |
Latest version of Cell Ranger with GRCh38 or GRCh38/mm10 build for corresponding sample preparation
snRNA seq was processed with intron-inclusive option
Assembly: GRCh38
Supplementary files format and content: CSV file with count matrix
|
| |
|
| Submission date |
Nov 12, 2022 |
| Last update date |
Apr 07, 2023 |
| Contact name |
Randy B Kang |
| E-mail(s) |
agarciaocana@coh.org
|
| Phone |
6262187939
|
| Organization name |
City of Hope Beckman Research Center
|
| Department |
Molecular and Cellular Endocrinology
|
| Lab |
Adolfo-Garcia Ocana
|
| Street address |
1500 E Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE217837 |
Single Cell RNA / Single Nucleus RNA Sequencing Data of Human Pancreaitc Islet |
|
| Relations |
| BioSample |
SAMN31700861 |
| SRA |
SRX18243046 |
