|
Status |
Public on Apr 07, 2023 |
Title |
Human Pancreatic Islet scRNA 2 |
Sample type |
SRA |
|
|
Source name |
Pancreatic Islet
|
Organism |
Homo sapiens |
Characteristics |
tissue: Pancreatic Islet condition: Non-diabetic Normal
|
Growth protocol |
serum free culture medium in non-adhesive culture plate in humidified atmosphere with 5% CO2 at 37C
|
Extracted molecule |
total RNA |
Extraction protocol |
scRNA was filtered with Miltenyi Biotec’s dead cell removal kit snRNA and graft snRNA extraction was done by Minute single nucleus isolation kit (cat#SN-047)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
integrated matrix
|
Data processing |
Latest version of Cell Ranger with GRCh38 or GRCh38/mm10 build for corresponding sample preparation snRNA seq was processed with intron-inclusive option Assembly: GRCh38 Supplementary files format and content: CSV file with count matrix
|
|
|
Submission date |
Nov 12, 2022 |
Last update date |
Apr 07, 2023 |
Contact name |
Randy B Kang |
E-mail(s) |
agarciaocana@coh.org
|
Phone |
6262187939
|
Organization name |
City of Hope Beckman Research Center
|
Department |
Molecular and Cellular Endocrinology
|
Lab |
Adolfo-Garcia Ocana
|
Street address |
1500 E Duarte Rd
|
City |
Duarte |
State/province |
CA |
ZIP/Postal code |
91010 |
Country |
USA |
|
|
Platform ID |
GPL24676 |
Series (1) |
GSE217837 |
Single Cell RNA / Single Nucleus RNA Sequencing Data of Human Pancreaitc Islet |
|
Relations |
BioSample |
SAMN31700861 |
SRA |
SRX18243046 |