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Sample GSM673684 Query DataSets for GSM673684
Status Public on Feb 11, 2011
Title Ferret whole blood with CFA plus vaccine immunizonion on day 1, biological rep1
Sample type RNA
 
Source name Ferret
Organism Mustela putorius furo
Characteristics tissue: Whole Blood
treatment group: with CFA plus vaccine immunizonion on day 1
Treatment protocol Ferrets were immunized with each adjuvanted vaccine through intramuscular rout by 200ul total volume in one leg.  At day 0 and day 1 post immunization, whole blood was collected in Paxgene tube for later RNA extraction.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA from lung was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from ferret whole blood with CFA plus vaccine immunizonion on day 1
Data processing The data were analyzed with PLIER using Expression Console Version 1.1 (Affymetrix) default analysis settings.
 
Submission date Feb 11, 2011
Last update date Feb 11, 2011
Contact name Longsi Ran
E-mail(s) lran@uhnresearch.ca
Phone 416-581-7607
Organization name Toronto General Research Institute
Department Division of Experimental Therapeutics
Lab Dr. David J Kelvin
Street address 101 College Street
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL3738
Series (1)
GSE27248 Molecular Characterization of In Vivo Adjuvant Activity in Ferrets Vaccinated against Influenza Virus

Data table header descriptions
ID_REF
VALUE PLIER-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 292.2276
AFFX-BioB-5_at 376.484
AFFX-BioB-M_at 382.3734
AFFX-BioC-3_at 698.5943
AFFX-BioC-5_at 608.9928
AFFX-BioDn-3_at 2878.675
AFFX-BioDn-5_at 1465.793
AFFX-Cfa-actin-3_s_at 6749.037
AFFX-Cfa-actin-5_at 6359.099
AFFX-Cfa-actin-5_s_at 3535.374
AFFX-Cfa-actin-M_x_at 8166.012
AFFX-Cfa-ef1a-3_x_at 8239.717
AFFX-Cfa-ef1a-5_at 15.43262
AFFX-Cfa-ef1a-M_x_at 2521.029
AFFX-Cfa-gapdh-3_x_at 144.9668
AFFX-Cfa-gapdh-5_x_at 114.8614
AFFX-Cfa-gapdh-M_x_at 21.55598
AFFX-Cfa-r2-Bs-dap-3_at 11.37533
AFFX-Cfa-r2-Bs-dap-5_at 11.61678
AFFX-Cfa-r2-Bs-dap-M_at 3.185109

Total number of rows: 43035

Table truncated, full table size 1231 Kbytes.




Supplementary file Size Download File type/resource
GSM673684.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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