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Sample GSM67370

Query DataSets for GSM67370

Status

Public on Sep 26, 2005

Title

del337TR replicate 2

Sample type

RNA

Source name

Mouse heart

Organism

Mus musculus

Characteristics

deletion in 337TR

Extracted molecule

total RNA

Extraction protocol

Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.

Label

biotin

Label protocol

Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

deletion in 337TR

Data processing

Robust Multichip Analysis (RMA)

Submission date

Aug 05, 2005

Last update date

Sep 26, 2005

Contact name

Norman Buroker

E-mail(s)

Norman.Buroker@seattlechildrens.org

Organization name

Seattle Children's Hospital

Lab

Portman

Street address

4800 Sand Point Way N.E.

City

Seattle

State/province

ZIP/Postal code

98105

Country

USA

Platform ID

GPL1261

Series (1)

GSE3067

Resistance to thyroid hormone (RTH) and PPARa activation

Data table header descriptions
ID_REF
VALUE	MAS5-calculated Signal intensity
ABS_CALL	the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE	'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	262.1	P	0.000857
AFFX-BioB-M_at	403.5	P	0.000297
AFFX-BioB-3_at	197.8	P	0.000857
AFFX-BioC-5_at	558.1	P	0.000169
AFFX-BioC-3_at	492.7	P	0.000052
AFFX-BioDn-5_at	567.4	P	0.000044
AFFX-BioDn-3_at	3325.7	P	0.000169
AFFX-CreX-5_at	8523.2	P	0.000052
AFFX-CreX-3_at	8931.3	P	0.000044
AFFX-DapX-5_at	16.6	A	0.262809
AFFX-DapX-M_at	35.1	A	0.250796
AFFX-DapX-3_at	48.2	A	0.18511
AFFX-LysX-5_at	2.2	A	0.824672
AFFX-LysX-M_at	35.6	A	0.5
AFFX-LysX-3_at	20	A	0.131361
AFFX-PheX-5_at	1613.8	P	0.000044
AFFX-PheX-M_at	2060.1	P	0.000052
AFFX-PheX-3_at	3860.8	P	0.000044
AFFX-ThrX-5_at	10.7	A	0.659339
AFFX-ThrX-M_at	17.3	A	0.544587

Total number of rows: 45101

Table truncated, full table size 1204 Kbytes.

Supplementary data files not provided