|
| Status |
Public on Sep 26, 2005 |
| Title |
Control with WY14643 replicate 5 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse heart
|
| Organism |
Mus musculus |
| Characteristics |
Control treated with WY14643
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total mRNA was extracted from the frozen heart tissue using the RNeasy fibrous tissue Kit from QIAGEN (Chatsworth, CA). Contaminating DNA was removed by deoxyribonuclease I digestion of the RNA preparation when still bound to the column. An average of 27ug of total RNA was obtained from 20mg of frozen mouse heart tissue.
|
| Label |
biotin
|
| Label protocol |
Approximately 5 µg of total RNA was processed to produce biotinylated cRNA targets.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Control treated with WY14643
|
| Data processing |
Robust Multichip Analysis (RMA)
|
| |
|
| Submission date |
Aug 05, 2005 |
| Last update date |
Sep 26, 2005 |
| Contact name |
Norman Buroker |
| E-mail(s) |
Norman.Buroker@seattlechildrens.org
|
| Organization name |
Seattle Children's Hospital
|
| Lab |
Portman
|
| Street address |
4800 Sand Point Way N.E.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98105 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE3067 |
Resistance to thyroid hormone (RTH) and PPARa activation |
|
