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Sample GSM6743921 Query DataSets for GSM6743921
Status Public on Nov 15, 2023
Title "spike for sgEts1_1"_ day 7 1
Sample type SRA
Source name sgRNA transduced OT-I cells
Organism Mus musculus
Characteristics cell type: sgRNA transduced OT-I cells
transduced: sgNTC (‘spike for sgEts1’)
strain: C57BL/6
Growth protocol OT-I cells transduced with indicatd sgRNAs were sorted from B16-OVA tumor after adoptive transfer
Extracted molecule total RNA
Extraction protocol CD8+ OT-I T cells were sorted from the dual-color transfer system at day 7 post adoptive transfer from B16-OVA tumor and loaded onto the Chromium Controller according to their respective cell counts to target 9000 single cell GEMs (gel beads in emulsion) per sample.
Libraries were prepared using the Chromium Single Cell 3’ v3 Library and Gel Bead Kit (10x Genomics)
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina HiSeq 4000
Data processing The Cell Ranger 6.0 Single-Cell Software Suite (10x Genomics) was implemented to process the raw sequencing data from the Illumina HiSeq run.
This pipeline performed de-multiplexing, alignment (mm10), and barcode processing to generate gene-cell matrices used for downstream analysis.
Assembly: mm10
Supplementary files format and content: cell ranger count matrix for single cells in each sample
Submission date Nov 19, 2022
Last update date Nov 15, 2023
Contact name Hongbo Chi
Organization name St Jude Children's Research Hospital
Department Immunology
Street address 262 Danny Thomas Place
City Memphis
State/province TN
ZIP/Postal code 38105
Country USA
Platform ID GPL21103
Series (2)
GSE216800 Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer
GSE218372 Single-cell CRISPR screens in vivo map T cell fate regulomes in cancer [Ets1 scRNAseq]
BioSample SAMN31800322
SRA SRX18319180

Supplementary file Size Download File type/resource
GSM6743921_S1_sgNTC.tar.gz 62.7 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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