|
| Status |
Public on Apr 06, 2023 |
| Title |
Blastoid_4CL, scRNAseq |
| Sample type |
SRA |
| |
|
| Source name |
Monkey blastoids
|
| Organism |
Macaca fascicularis |
| Characteristics |
cell type: Monkey blastoids
age: D7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Monkey blastoids were picked up manually by mouth pipette. Then blastoids were washed with PBS containing 0.05% BSA and dissociated with trypsin-EDTA (0.25%) (Gibco) at 37 °C in 5% CO2 for 10 min. The dissociation was terminated by PBS with 15% FBS and centrifuged the sample at 300g for 5 min. Cell pellets were resuspended in PBS containing 0.05% BSA, filtered through a 40-μm cell strainer and counted.
Single-cell suspensions were loaded into a 10X Genomics Chromium Chip following the manufacturer’s instructions 48 (10X Genomics, Chromium Next GEM Single Cell 3ʹ GEM, Library and Gel Bead Kit v.3.1).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
10x Genomics
|
| Data processing |
The 10X Genomics single-cell data were processed with common Cell Ranger pipeline (v.6.1.2) to generate the expression matrix. The number of single cells in the assay was determined by the Cell Ranger pipeline with the expecting cell number parameter (8000 cells). The reference and gene annotations Macaca fascicularis genome (Macaca_fascicularis_5.0) were prepared as Cell Ranger pipeline.
Assembly: Macaca_fascicularis_5.0
Supplementary files format and content: Comma-separated values files and matrix files
|
| |
|
| Submission date |
Nov 19, 2022 |
| Last update date |
Apr 06, 2023 |
| Contact name |
yuan qing zhu |
| E-mail(s) |
zhuqingyuan@mail.tsinghua.edu.cn
|
| Organization name |
Tsinghua University
|
| Street address |
Zhongguancun north street
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
|
| Platform ID |
GPL28212 |
| Series (1) |
| GSE218375 |
Cynomolgus monkey embryo model captures gastrulation and early pregnancy |
|
| Relations |
| BioSample |
SAMN31801968 |
| SRA |
SRX18321254 |
