|
| Status |
Public on Feb 15, 2011 |
| Title |
gd cell activation+6h,donor 2 |
| Sample type |
RNA |
| |
|
| Source name |
human blood sample, freshly isolated and purified, 6 hours after in vitro activation
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: peripheral blood lymphocytes isolated and activated in vitro for 6 hrs
cell type: TCRVgamma9+ gamma delta T cells
treatment: BrHPP/IL2
time: 6 h
individual: donor 2
|
| Treatment protocol |
Cells were activated by adding BrHPP (500 nM) ad day 0.
|
| Growth protocol |
Cells were cultured in complete RPMI 1640 medium containing 2 mM L-glutamine, 100 µg/ml streptomycin, 100 IU/ml penicillin and 1 mM sodium-pyruvate supplemented with 10% fetal calf serum and recombinant human IL-2 (100 IU/ml) added every three days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1-3 µg total RNA from human cells, amplified and labeled following the one-Cycle Target Labeling protocol (Affymetrix, Santa Clara, CA, USA).
|
| |
|
| Hybridization protocol |
Labeled complementary RNA (cRNA) from these samples was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.
|
| Scan protocol |
The chips were then washed, scanned on the Affymetrix Fluidics Station 400 and analyzed with GeneChip Operating Software.
|
| Description |
Gene expression data from purified TCRVgamma9 positive cells
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 14, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Jean Jacques FOURNIE |
| E-mail(s) |
jean-jacques.fournie@inserm.fr
|
| Phone |
+33619900602
|
| Organization name |
INSERM
|
| Department |
oncology
|
| Lab |
Cancer Research Center of Toulouse
|
| Street address |
2 av Hubert Curien
|
| City |
Toulouse |
| ZIP/Postal code |
31037 |
| Country |
France |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27291 |
Microarray expression data from human TCRVg9-positive gamma delta T lymphocytes |
|
| Relations |
| Reanalyzed by |
GSE86362 |
