NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM675863 Query DataSets for GSM675863
Status Public on Jun 30, 2012
Title C.elegans_8days_1G3-2
Sample type RNA
 
Source name 1G
Organism Caenorhabditis elegans
Characteristics strain: N2 wild type
developmental stage: adult
Growth protocol Approximately 50 L1 larvae were grown in 2 mL M9. Worms were cultured in 12 mL S basal medium contained bacterial feeds for 8days.
Extracted molecule total RNA
Extraction protocol Worms were homogenized with four rounds of 20-second beating at 5000 rpm in a Micro Smash TM MS-100 bead beater with 3 minutes of cooling on ice between rounds. Total RNA was extracted using an Isogen RNA purification kit following the manufacturer’s protocol.
Label Cy3
Label protocol Total RNA was amplified using the Low Input Quick Amp Labeling Kit protocol. Cy3 labeled cRNA was produced according to manufacturer's protocol.
 
Hybridization protocol Labeled cRNA (1.65 µg/sample) was hybridized to a C. elegans oligo microarray using a gene expression hybridization kit at 65 °C for 17 hours.
Scan protocol Array were scanned with an Agilent Scanner and processed with Agilent Feature Extraction Software.
Data processing Microarray data were analyzed using Gene Spring software (Agilent Technologies). Normalized data were produced by "Normalize to 75 percentile sift protocol’’ and "baseline to median of control sample protocol’’. Arrays were filtered on expression (20-100)th percentile in raw data.
 
Submission date Feb 16, 2011
Last update date Jun 30, 2012
Contact name Akira Higashibata
E-mail(s) higashibata.akira@jaxa.jp
Phone 050-3362-3898
Organization name Japan Aerospace Exploration Agency
Department Department of Space Biology and Microgravity Sciences
Lab ISS Science Project office
Street address 2-1-1,Sengen
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8505
Country Japan
 
Platform ID GPL11346
Series (1)
GSE27338 Microgravity effect on C. elegans N2/VC (CERISE, 8days)

Data table header descriptions
ID_REF
RAW raw data
VALUE GeneSpring normalized signal intensity

Data table
ID_REF RAW VALUE
A_12_P100000 21.040009 0.14642048
A_12_P100002 71.023315 -0.09395695
A_12_P100003 507.2212 -0.001100063
A_12_P100004 4259.9746 -0.03559923
A_12_P100005 154.9515 0.14269137
A_12_P100006 246.36191 -0.13980556
A_12_P100007 749.9064 -0.031476974
A_12_P100008 1163.6709 -0.049553394
A_12_P100011 30.46011 -0.49585056
A_12_P100012 576.17737 -0.1991148
A_12_P100014 14050.071 -0.078197956
A_12_P100016 188.22923 0.019564867
A_12_P100019 113.11371 0.052170277
A_12_P100020 162.55861 -0.00756979
A_12_P100021 17515.586 0.3937707
A_12_P100022 179.83768 0.083042145
A_12_P100023 2176.8008 0.07641411
A_12_P100024 2693.6482 0.06359291
A_12_P100025 2034.3667 -0.27515793
A_12_P100026 733.0118 0.12887287

Total number of rows: 34226

Table truncated, full table size 1147 Kbytes.




Supplementary file Size Download File type/resource
GSM675863.txt.gz 8.3 Mb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap