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Sample GSM675868 Query DataSets for GSM675868
Status Public on Jun 30, 2012
Title C.elegans_8days_GC2-2
Sample type RNA
 
Source name Ground control
Organism Caenorhabditis elegans
Characteristics strain: N2 wild type
developmental stage: adult
Growth protocol Approximately 50 L1 larvae were grown in 2 mL M9. Worms were cultured in 12 mL S basal medium contained bacterial feeds for 8days.
Extracted molecule total RNA
Extraction protocol Worms were homogenized with four rounds of 20-second beating at 5000 rpm in a Micro Smash TM MS-100 bead beater with 3 minutes of cooling on ice between rounds. Total RNA was extracted using an Isogen RNA purification kit following the manufacturer’s protocol.
Label Cy3
Label protocol Total RNA was amplified using the Low Input Quick Amp Labeling Kit protocol. Cy3 labeled cRNA was produced according to manufacturer's protocol.
 
Hybridization protocol Labeled cRNA (1.65 µg/sample) was hybridized to a C. elegans oligo microarray using a gene expression hybridization kit at 65 °C for 17 hours.
Scan protocol Array were scanned with an Agilent Scanner and processed with Agilent Feature Extraction Software.
Data processing Microarray data were analyzed using Gene Spring software (Agilent Technologies). Normalized data were produced by "Normalize to 75 percentile sift protocol’’ and "baseline to median of control sample protocol’’. Arrays were filtered on expression (20-100)th percentile in raw data.
 
Submission date Feb 16, 2011
Last update date Jun 30, 2012
Contact name Akira Higashibata
E-mail(s) higashibata.akira@jaxa.jp
Phone 050-3362-3898
Organization name Japan Aerospace Exploration Agency
Department Department of Space Biology and Microgravity Sciences
Lab ISS Science Project office
Street address 2-1-1,Sengen
City Tsukuba
State/province Ibaraki
ZIP/Postal code 305-8505
Country Japan
 
Platform ID GPL11346
Series (1)
GSE27338 Microgravity effect on C. elegans N2/VC (CERISE, 8days)

Data table header descriptions
ID_REF
RAW raw data
VALUE GeneSpring normalized signal intensity

Data table
ID_REF RAW VALUE
A_12_P100000 42.05057 1.3846054
A_12_P100002 28.34316 -1.1800542
A_12_P100003 326.34866 -0.3981061
A_12_P100004 3875.949 0.06730032
A_12_P100005 132.15143 0.1522615
A_12_P100006 321.47522 0.4833176
A_12_P100007 624.62897 -0.05599308
A_12_P100008 652.5178 -0.6449523
A_12_P100011 89.14663 1.2926035
A_12_P100012 708.9526 0.3392563
A_12_P100014 12247.505 -0.037092686
A_12_P100016 95.58981 -0.7188022
A_12_P100019 80.99657 -0.19047594
A_12_P100020 67.69082 -1.0323029
A_12_P100021 12038.344 0.09196329
A_12_P100022 142.8431 -0.010026932
A_12_P100023 2052.7344 0.23094654
A_12_P100024 2705.7798 0.30927086
A_12_P100025 1847.6426 -0.17485714
A_12_P100026 701.8714 0.30543804

Total number of rows: 34226

Table truncated, full table size 1133 Kbytes.




Supplementary file Size Download File type/resource
GSM675868.txt.gz 8.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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