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| Status |
Public on Dec 12, 2022 |
| Title |
Monkey_Spleen_Male_1002 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Macaca fascicularis |
| Characteristics |
tissue: Spleen
Sex: Male
treatment: untreated
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| Treatment protocol |
NA
NA
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| Growth protocol |
NA
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| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy mini kit (Qiagen). 200-300ng of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using TruSeq Stranded Total RNA Library Prep Kit Gold following manufacturer's protocols.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
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| Description |
Monkey_Spleen_Male_1002
Cyno_FPKM_table_Mar22.xlsx
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| Data processing |
ArrayStudio
Sequence reads were trimmed for adaptor sequence/low-quality sequence using ArrayStudio
Trimmed sequence reads were mapped to Mouse.B38, Rat.B6.0, Dog.CanFam3.1, and Macaca_fascicularis_5.0
Read count extraction and normalization were performed using ArrayStudio
Assembly: Mouse.B38, Rat.B6.0, Dog.CanFam3.1, and Macaca_fascicularis_5.0
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
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| Submission date |
Nov 29, 2022 |
| Last update date |
Dec 12, 2022 |
| Contact name |
Terry Van Vleet |
| Organization name |
AbbVie
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| Street address |
1 N Waukegan Rd
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| City |
North Chicago |
| State/province |
IL |
| ZIP/Postal code |
60064 |
| Country |
USA |
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| Platform ID |
GPL18648 |
| Series (1) |
| GSE219045 |
Preclinical Species Gene Expression Database: Development and Meta-Analysis |
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| Relations |
| BioSample |
SAMN31934834 |
| SRA |
SRX18425473 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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