NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM676996 Query DataSets for GSM676996
Status Public on Feb 18, 2011
Title MCF7-ERbeta cells, 1nM estradiol treated, 24 hours, replicate 1
Sample type RNA
 
Source name ER-beta expressing MCF7 cells
Organism Homo sapiens
Characteristics genotype/variation: estrogen-receptor beta expressing cells
agent: 1nM estradiol
cell line: MCF7
cell type: breast cancer cells
Treatment protocol All cell treatments were conducted in phenol red-free DMEM/F12 medium containing 10% triple charcoal stripped FBS and 1% antibiotic-antimycotic.
Growth protocol MCF7 cells were cultured in phenol red-free Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) medium containing 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) antibiotic-antimycotic (AA) solution in a humidified 37°C incubator with 5% CO2. MCF7 cells stably expressing ERβ were generated using an S-tagged-Flag-tagged ERβ expression construct (pIRES2-EGFP) and were cultured in identical medium containin 300 microL/mL G418.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol reagent.
Label Cy3
Label protocol Cy3
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description Replicate 1
Data processing The raw data was pre-processed by Illumina's beadstudio without backgroud subtraction and gene level profile was obtained. This data was exported, log2 ransformed and normalized using fast lowess normalization in R
 
Submission date Feb 17, 2011
Last update date Feb 18, 2011
Contact name John Hawse
E-mail(s) hawse.john@mayo.edu
Phone 507-284-4268
Organization name Mayo Clinic
Department Biochemistry and Molecular Biology
Street address 200 1st St. SW
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
 
Platform ID GPL6947
Series (1)
GSE27375 Gene expression profiles elicited by estradiol and endoxifen in MCF7 parental and ER-beta expressing breast cancer cells

Data table header descriptions
ID_REF
VALUE Gene level profile; log2 transformed, fast lowess normalized data in R
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1762337 6.508505305 0.9275362
ILMN_2055271 6.921289041 0.08503058
ILMN_1779670 6.684339088 0.6883858
ILMN_2321282 6.894594869 0.03100345
ILMN_1772582 6.767983614 0.3491436
ILMN_1735698 7.12457546 0.01844532
ILMN_1717783 6.842761463 0.1341336
ILMN_2359168 6.675932207 0.7096108
ILMN_1745607 6.548489952 0.8827404
ILMN_2136495 6.625486515 0.7338603
ILMN_2295559 6.775419064 0.215251
ILMN_1735045 8.173011553 0
ILMN_1680754 6.782329179 0.3069829
ILMN_1673870 6.704812644 0.570071
ILMN_1755321 7.72327974 0.001317523
ILMN_1698554 9.144028387 0
ILMN_1814092 6.878485635 0.1264822
ILMN_1760414 6.914300128 0.07641634
ILMN_1676336 7.396159235 0.00010502
ILMN_1752884 6.669726228 0.6218709

Total number of rows: 37780

Table truncated, full table size 1251 Kbytes.




Supplementary file Size Download File type/resource
GSM676996_Sample9_5050496034_I_Grn.idat.gz 2.2 Mb (ftp)(http) IDAT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.