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Status |
Public on Feb 18, 2011 |
Title |
MCF7-ERbeta cells, 1nM estradiol treated, 24 hours, replicate 1 |
Sample type |
RNA |
|
|
Source name |
ER-beta expressing MCF7 cells
|
Organism |
Homo sapiens |
Characteristics |
genotype/variation: estrogen-receptor beta expressing cells agent: 1nM estradiol cell line: MCF7 cell type: breast cancer cells
|
Treatment protocol |
All cell treatments were conducted in phenol red-free DMEM/F12 medium containing 10% triple charcoal stripped FBS and 1% antibiotic-antimycotic.
|
Growth protocol |
MCF7 cells were cultured in phenol red-free Dulbecco’s modified Eagle’s medium/F12 (DMEM/F12) medium containing 10% (v/v) Fetal Bovine Serum (FBS) and 1% (v/v) antibiotic-antimycotic (AA) solution in a humidified 37°C incubator with 5% CO2. MCF7 cells stably expressing ERβ were generated using an S-tagged-Flag-tagged ERβ expression construct (pIRES2-EGFP) and were cultured in identical medium containin 300 microL/mL G418.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Trizol reagent.
|
Label |
Cy3
|
Label protocol |
Cy3
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
Replicate 1
|
Data processing |
The raw data was pre-processed by Illumina's beadstudio without backgroud subtraction and gene level profile was obtained. This data was exported, log2 ransformed and normalized using fast lowess normalization in R
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|
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Submission date |
Feb 17, 2011 |
Last update date |
Feb 18, 2011 |
Contact name |
John Hawse |
E-mail(s) |
hawse.john@mayo.edu
|
Phone |
507-284-4268
|
Organization name |
Mayo Clinic
|
Department |
Biochemistry and Molecular Biology
|
Street address |
200 1st St. SW
|
City |
Rochester |
State/province |
MN |
ZIP/Postal code |
55905 |
Country |
USA |
|
|
Platform ID |
GPL6947 |
Series (1) |
GSE27375 |
Gene expression profiles elicited by estradiol and endoxifen in MCF7 parental and ER-beta expressing breast cancer cells |
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