|
| Status |
Public on Dec 09, 2022 |
| Title |
Blood_before_intervention_Patient5 |
| Sample type |
genomic |
| |
|
| Source name |
Whole Blood
|
| Organism |
Homo sapiens |
| Characteristics |
patient: Patient5
time point: Timepoint1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
The DNA from the blood samples at first timepoint was extracted using Invisorb® Spin Blood Mini Kit (Stratec Biomedical AG, Germany) following the manufacturer’s instructions and salting out method from the second and third timepoint.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
Bisulfite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human MethylationEPIC Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
|
| Description |
DNA extracted from whole blood from patient with NAFLD
|
| Data processing |
Data were processed in R, normalized using BMIQ normalization.
|
| |
|
| Submission date |
Dec 02, 2022 |
| Last update date |
Dec 10, 2022 |
| Contact name |
Tomasz K Wojdacz |
| E-mail(s) |
tomasz.wojdacz@pum.edu.pl
|
| Organization name |
Pomeranian Medical University
|
| Department |
Independent Clinical Epigenetics Laboratory
|
| Street address |
Unii Lubelskiej 1
|
| City |
Szczecin |
| State/province |
Zachodniopomorskie |
| ZIP/Postal code |
71-252 |
| Country |
Poland |
| |
|
| Platform ID |
GPL23976 |
| Series (1) |
| GSE219217 |
Mediterranean diet and fiber supplementation change the whole blood DNA methylation pattern of NAFLD patients |
|
