|
| Status |
Public on Dec 19, 2011 |
| Title |
Sample-1027-Before |
| Sample type |
RNA |
| |
|
| Source name |
Serum, baseline
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
disease state: healthy
tissue: serum
diet intervention stage: before
individual: 1027
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA, including miRNA from plasma, was isolated using the miRNeasy kit (Qiagen) with minor modifications. The quality and quantity of the RNA was evaluated by 260/280 ratio using NanoDrop spectrophotometry (NanoDrop ND-1000 Technologies Inc.) and the Agilent 2100 Bioanalyzer (Agilent Technologies).
|
| Label |
biotin
|
| Label protocol |
Two hundred ng of total RNA from each sample were labeled according to the manufacturer's recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| |
|
| Hybridization protocol |
Labeled RNA were hybridized on Human v2 MicroRNA Expression BeadChips (Cat. no. MI-102–1024; Illumina) according to the manufacturer's recommendations (Illumina MicroRNA Expression Profiling Assay Guide).
|
| Scan protocol |
Illumina BeadArray Reader with standard scanning protocol.
|
| Description |
DBH00110
|
| Data processing |
The data were normalized using quantile normalization with the lumi package in R.
|
| |
|
| Submission date |
Feb 23, 2011 |
| Last update date |
Dec 19, 2011 |
| Contact name |
Dan Wang |
| E-mail(s) |
wangdan412@gmail.com
|
| Organization name |
RPCI
|
| Street address |
Elm & Carlton Streets
|
| City |
buffalo |
| State/province |
NY |
| ZIP/Postal code |
14263 |
| Country |
USA |
| |
|
| Platform ID |
GPL8179 |
| Series (1) |
| GSE27474 |
Reduction of dietary glycemic load modifies expression of several miRNAs associated with energy balance and cancer pathways in premenopausal women |
|
