|
| Status |
Public on Jan 17, 2024 |
| Title |
CD34 treated with 0.5 mM of Denatonium biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
umbilical cord blood derived CD34+ cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: umbilical cord blood (UCB)-derived CD34+ cells
treatment: 0.5 mM of Denatonium
|
| Treatment protocol |
Denatonium Benzoate (DEN) treatment
|
| Growth protocol |
UCB-derived CD34+ cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), 1% Penicillin/Streptomycin (Pen/Strep) (MP Biomedicals), 1% Hepes buffer , SCF (50 ng/ml), IL-3 (50 ng/ml), GM-CSF (10 ng/ml) and maintained at 37°C in a humidified 5% CO2 incubator, with or without increasing doses of Denatonium Benzoate (DEN)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using a Rneasy Micro kit,according to the manufacturer’s instructions
|
| Label |
Biotin
|
| Label protocol |
The fragmented cDNA is labeled by terminal deoxynucleotidyl transferase (TdT), according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
The cDNA fragments were hybridized on cartridge array using the GeneChipTM Hybridization Oven 645 set ON at 60 rpm and 45°C.
|
| Scan protocol |
The microarrays were finally scanned on a GeneChipTM Scanner 3000 System (Affymetrix).The fluorescent signals relative to hybridized probes were obtained as DAT files and were converted into CEL digital signal by Affymetrix GeneChip Command Console (AGCC) software
|
| Description |
Gene expression data from umbilical cord blood (UCB)-derived CD34+ cells after denatonium benzoate treatment
|
| Data processing |
CEL files' raw data were normalized using Robust Multi-array Average normalization (RMA) and log-transformed using Oligo R package.Normalized data were annotated by pd.clariom.s.human
|
| |
|
| Submission date |
Dec 06, 2022 |
| Last update date |
Jan 17, 2024 |
| Contact name |
Maria Antonella Laginestra |
| E-mail(s) |
mariaantonella.laginestra@ior.it
|
| Organization name |
IRCCS Istituto Ortopedico Rizzoli, Bologna, Italy
|
| Department |
Experimental Oncology Laboratory
|
| Street address |
Via di Barbiano 1/10
|
| City |
Bologna |
| ZIP/Postal code |
40136 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE220210 |
THE BITTER TASTE RECEPTOR AGONIST DENATONIUM INHIBITS STEMNESS CHARACTERISTICS IN HEMATOPOIETIC STEM CELLS |
|
