|
| Status |
Public on May 01, 2023 |
| Title |
Rabbit_Aorta_Atherosclerosis_no2 |
| Sample type |
RNA |
| |
|
| Source name |
Aorta
|
| Organism |
Oryctolagus cuniculus |
| Characteristics |
sample type: Male, NZW, abdonimal aorta, stable advanced atherosclerosis
|
| Treatment protocol |
After surgical intervention the rabbits with Atherosclerosis were fed with 0.30% high cholesterol diet and after 8 weeks were switched to a maintance diet of 0.15% high cholesterol diet for a remainig of 12 weeks
|
| Growth protocol |
The rabbits were housed with enrichment and had ad libitum of chow or High cholesterol diet, depending on their treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
We sacrificed the rabbits, and surgically removed the adbominal aortas. We flushed them with saline and put cut sectios into RNAlater for storage overnight in the fridge. Then the RNAlater was removed and the tissue was tored at -20C until extraction of RNA.
|
| Label |
SYBR green
|
| Label protocol |
Total RNA was extracted with TRIzol® Reagent and reverse-transcribed into cDNA using the RT2 First Strand Kit QIAGEN. And plated out on PCR array plates. Plates were read on the Mx3000P real-time PCR system (Stratagene, CA, USA) with paramters by 1 cycle 10 minutes of 95C and then 40 cycles of 15s at 95C and 1 min at 60C
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Atherosclerotic
|
| Data processing |
By using QIAGEN GeneGlobe analysis program
|
| |
|
| Submission date |
Dec 12, 2022 |
| Last update date |
May 01, 2023 |
| Contact name |
Constance E Grandjean |
| Organization name |
University of Copenhagen
|
| Department |
Cluster for Molecular Imaging
|
| Street address |
Blegdamsvej 3
|
| City |
CPH |
| ZIP/Postal code |
2100 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL32943 |
| Series (1) |
| GSE220754 |
QIAGEN RT2 profiler Rabbit Wound Healing |
|
