|
| Status |
Public on Apr 04, 2023 |
| Title |
Sterne mock treated 2h replicate 3 |
| Sample type |
SRA |
| |
|
| Source name |
vegetative
|
| Organism |
Bacillus anthracis |
| Characteristics |
strain: Sterne
cell type: vegetative
genotype: pXO1+/pXO2-
treatment: 2 h mock
|
| Treatment protocol |
mock treated or 10 ug anthrose treatment
|
| Growth protocol |
Growth in BHI
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Zymo Direct-zol RNA Miniprep Plus kit including the optional bead beating steps as well as the on column DNAse treatment.
NEBNext Ultra II RNA Library Prep Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Neg3_2h
|
| Data processing |
base calling
filtering
Bowtie
cufflink
cuffdiff
Assembly: GCF_000832645.1
Supplementary files format and content: tuxedo output table
|
| |
|
| Submission date |
Dec 12, 2022 |
| Last update date |
Apr 04, 2023 |
| Contact name |
Michael H Norris |
| E-mail(s) |
mhnorris@ufl.edu
|
| Organization name |
University of Florida
|
| Street address |
2055 Mowry Rd
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
| |
|
| Platform ID |
GPL31186 |
| Series (1) |
| GSE220794 |
Beyond the spore: the exosporium sugar anthrose impacts vegetative Bacillus anthracis gene regulation in cis and trans |
|
| Relations |
| BioSample |
SAMN32180061 |
| SRA |
SRX18658924 |
