|
| Status |
Public on Mar 15, 2013 |
| Title |
kgb-1, Fast , rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
kgb-1, Fast
|
| Organism |
Caenorhabditis elegans |
| Characteristics |
strain background: N2
genotype/variation: kgb-1 mutant
treatment: Fast
|
| Treatment protocol |
Worms were subject to Fed or Fasted conditions for indicated time and total RNA was extracted.
|
| Growth protocol |
Worms were fed til day 2 of adult.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were prepared by using Trizol (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the Affymetrix protocol (two-Cycle Target Labeling Assays) from 100 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on C. elegans Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Styation 450.
|
| Scan protocol |
GeneChips were scanned using an Affymetrix GeneChip Scanner.
|
| Description |
Gene expression data from 4 day adult
|
| Data processing |
The data were analyzed with PLIER algorithm using GeneSpring GX 11.0.2.
|
| |
|
| Submission date |
Mar 04, 2011 |
| Last update date |
Mar 15, 2013 |
| Contact name |
Eisuke Nishida |
| E-mail(s) |
nishida@lif.kyoto-u.ac.jp
|
| Phone |
+81-75-753-4230
|
| Organization name |
Graduate School of Biostudies, Kyoto University
|
| Department |
Department of Cell and Developmental Biology
|
| Street address |
Kitashirakawa, Sakyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
606-8502 |
| Country |
Japan |
| |
|
| Platform ID |
GPL200 |
| Series (1) |
| GSE27677 |
A conserved JNK/AP-1 module is a key mediator of intermittent fasting-induced longevity in C. elegans |
|
