|
| Status |
Public on Mar 08, 2011 |
| Title |
Wild Type MAECs: 1103ZG0005 |
| Sample type |
RNA |
| |
|
| Source name |
Wild Type MAECs: 1103ZG0005
|
| Organism |
Mus musculus |
| Characteristics |
cell type: mouse aorta endothelial cells
genotype/variation: Wild type
strain: C57BL/6J
|
| Growth protocol |
MAECs grown to 80% confluence in six-well plates were made quiescent in serum-free DMEM for 12 h and then cultured in 10% FBS DMEM for 12 h.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Trizol® reagent and purified by Rneasy Mini kit.
|
| Label |
biotin
|
| Label protocol |
Affymetrix standard protocol was used.
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol was used.
|
| Scan protocol |
GeneChips were scanned using GeneArray Scanner 3000 and analyzed with the Affymetrix GeneChip Command Console.
|
| Data processing |
The data were analyzed and normalized with Affymetrix Expression Console software (program-version: 1.1.2800.19935, affymetrix-algorithm-name = rma-gene-default).
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
Mar 08, 2011 |
| Contact name |
Xinghua Lin |
| E-mail(s) |
xlin@mmc.edu
|
| Phone |
615-327-6537
|
| Fax |
615-327-6537
|
| Organization name |
Meharry Medical College
|
| Department |
Physiology
|
| Street address |
1005 D. B. Todd Jr. Blvd
|
| City |
Nashville |
| State/province |
Tennessee |
| ZIP/Postal code |
37208 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27701 |
Genes Express in Catalase Overexpression MAECs |
|
