|
| Status |
Public on May 19, 2011 |
| Title |
CD34(-)KSL hematopoietic stem cells, biological replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
CD34(-)KSL hematopoietic stem cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD34(-)KSL hematopoietic stem cells
genetic background: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using an ISOGEN reagent (Nippon Gene).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared from the purified total RNA equivalent to 3,000 cells with a Two-Cycle cDNA Synthesis Kit and 3'-Amplification Reagents for IVT Labeling (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 μg of cRNA were hybridized for 16 hr at 45˚C on GeneChip Mouse Genome 430 2.0 Array (Affymetrix). Arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
CD34(-)KSL hematopoietic stem cells purified by cell sorting
|
| Data processing |
The data were analyzed with GeneChip Operating Softwere version 1.4 (GCOS1.4, Affymetrix) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
May 19, 2011 |
| Contact name |
Hitoshi Ichikawa |
| E-mail(s) |
hichikaw@ncc.go.jp
|
| Phone |
+81-3-3542-2511
|
| Fax |
+81-3-3248-1631
|
| Organization name |
National Cancer Center Research Institute
|
| Lab |
Genetics Division
|
| Street address |
5-1-1 Tsukiji, Chuo-ku
|
| City |
Tokyo |
| ZIP/Postal code |
104-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE27785 |
Gene expression profile of mouse hematopoietic stem cells |
| GSE27787 |
Hematopoietic cells and stem cells |
|
