|
| Status |
Public on Jul 01, 2011 |
| Title |
Tet2 KO GMP rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA, Tet2 KO GMP
|
| Organism |
Mus musculus |
| Characteristics |
genetic background: B6/129
genotype: Tet2 KO
cell type: Lineage negative, c-Kit positive and Sca1 (LSK) negative FCgammaReceptor High CD34 positive expressing bone marrow progenitors GMP
|
| Treatment protocol |
LSK and CMP cells were sorted from Tet2+/+ Mx+, Tet-/-Mx+ littermate mice 4 weeks post deletion via pIC injection. Additionally cells were obtained from serial replating of CD150+ sorted Tet2 deficient cells.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNEasy Plus Kit was used to extract total cellular RNA according to the manufacturer's protocol, and the RNA was amplified and converted into cDNA using the Ovation Amplification v2 and Biotin cDNA Biotin systems
|
| Label |
biotin
|
| Label protocol |
5 ng total RNA was amplified by Nugen Ovation RNA amplification system V2 and the resulting cDNA biotin-labeled using the FL-Ovation™ cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
3.75 mcg of biotin-labeled cDNA were hybridized for 16 hrs onto the GeneChip Mouse Genome 430 2.0 Arrays (Affymetrix)
|
| Scan protocol |
Affymetrix 7G GeneArray scanner
|
| Description |
Tet2 deficient Lineage negative, c-Kit positive and Sca1 (LSK) negative FCgammaReceptor High CD34 positive expressing bone marrow progenitors-repeat2
Gene expression data from Tet2 deficient Lineage negative, c-Kit positive and Sca1 (LSK)negative FCgammaReceptor High CD34 positive expressing bone marrow progenitors-repeat2
|
| Data processing |
Feature intensity was extracted by GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using dChip program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Mar 07, 2011 |
| Last update date |
Jul 01, 2011 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
zavadilj@iarc.fr
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27816 |
Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation |
|
