|
| Status |
Public on Jan 31, 2023 |
| Title |
Catagen SHFs in December, male (bio rep 2) |
| Sample type |
SRA |
| |
|
| Source name |
Secondary hair follicles from goat skin
|
| Organism |
Capra hircus |
| Characteristics |
tissue: Secondary hair follicles from goat skin
Sex: male
age: 1.5 years
month: December
growth stage: Catagen
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the secondary hair follicles using RNAiso Plus (TRIzol method, TaKaRa Bio, Dalian, China).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 4000 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, and mapped to goat (Capra hircus) transcriptomes (GCF_001704415.1_ARS1) using Kallisto (v.0.46.1) with parameters -b 100. The gene abundances were obtained by kallisto-sleuth (v.0.30.0) pipeline in TPM (transcripts per million) (Bray NL et al. 2016).
Assembly: GCF_001704415.1_ARS1
Supplementary files format and content: tab-delimited text files include TPM values for each Sample
|
| |
|
| Submission date |
Dec 20, 2022 |
| Last update date |
Feb 01, 2023 |
| Contact name |
Huiling Xue |
| E-mail(s) |
hxuemail@126.com
|
| Organization name |
Shenyang Agricultural University
|
| Street address |
120 Dongling Road,110866 Shenyang,China
|
| City |
Shenyang |
| ZIP/Postal code |
110866 |
| Country |
China |
| |
|
| Platform ID |
GPL22146 |
| Series (1) |
| GSE221472 |
Molecular Signatures and Functional Analysis of Secondary Hair Follicles in Cashmere Goat During Annual Cashmere Growth |
|
| Relations |
| BioSample |
SAMN32338325 |
| SRA |
SRX18798088 |
