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Status |
Public on Feb 15, 2023 |
Title |
3D4/21, untreated, RNA-Seq, rep2 [PolyA-enrich.00h_r2] |
Sample type |
SRA |
|
|
Source name |
3D4/21
|
Organism |
Sus scrofa |
Characteristics |
cell line: 3D4/21 genotype: Wild-type treatment: none; untreated
|
Treatment protocol |
GSK126 was added to RPMI 1640 medium to make its final concentration as 5 μmol/L. The same volume of DMSO was added for the untreated control group. The control group and GSK126 treated group are collected for subsequent experiments.
|
Growth protocol |
Porcine 3D4/21 alveolar macrophages were cultured in 1640 medium with 10% fetal bovine serum and 1% penicillin-streptomycin (100×), incubated in a constant temperature incubator with 37 ℃, 5% CO2 and saturated humidity.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA for porcine alveolar macrophage was extracted using the FastPure® Cell/Issue Total RNA Isolation Kit BOX2 Kit(Vazyme). RNA-Seq library is constructed by BGI company using the PolyA-enrichment protocol.
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|
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
MGISEQ-2000RS |
|
|
Data processing |
Raw reads were trimmed with Trim Galore v0.6.4. Transcript Per Million (TPM) values were calculated with RSEM v1.3.2 with default settings. Assembly: Sscrofa11 Supplementary files format and content: tab-delimited text files include TPM and FPKM values for each sample
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|
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Submission date |
Dec 21, 2022 |
Last update date |
Feb 15, 2023 |
Contact name |
Z LL |
E-mail(s) |
MZ120201482@stu.yzu.edu.cn
|
Phone |
13283789136
|
Organization name |
yangzhouaxue
|
Street address |
shuangqiaojiedao
|
City |
yangzhou |
ZIP/Postal code |
225009 |
Country |
China |
|
|
Platform ID |
GPL32965 |
Series (2) |
GSE221477 |
Inhibition of EZH2 causes retrotransposon derepression and immune activation in porcine lung alveolar macrophages [polyA-enriched RNA-seq] |
GSE221479 |
Inhibition of EZH2 causes retrotransposon derepression and immune activation in porcine lung alveolar macrophages. |
|
Relations |
BioSample |
SAMN32340987 |
SRA |
SRX18799780 |