 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 07, 2023 |
| Title |
K9 |
| Sample type |
SRA |
| |
|
| Source name |
AngII/PE treated female
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Whole LV myocardium
genotype: WT C57Bl6J/129X1 hybrid
treatment: AngII/PE infusion for 3 days
Sex: female
time: 3 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Ambion PureLink RNA minikit, lot 2379554, following manufacturer's protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
RNA-Seq reads were trimmed by cutadapt first, then trimmed read were aligned against mouse genome (mm10) using STAR v2.4.2a. Gene counts were derived from the number of uniquely aligned unambiguous reads by Subread: featureCount with mouse GENCODE gene annotation (M15).
All gene-level transcript counts were then imported into DESeq2, and normalization method was used to adjust for differences in library size
Assembly: mm10
Supplementary files format and content: xlsx
|
| |
|
| Submission date |
Dec 21, 2022 |
| Last update date |
Jun 07, 2023 |
| Contact name |
Tiandao Li |
| Organization name |
Washington University
|
| Street address |
4444 Forest Park Ave
|
| City |
St Louis |
| State/province |
MO |
| ZIP/Postal code |
63108 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE221502 |
Characterization of a robust mouse model for heart failure with preserved ejection fraction |
|
| Relations |
| BioSample |
SAMN32347894 |
| SRA |
SRX18804613 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
