|
| Status |
Public on Jun 22, 2011 |
| Title |
Frontal cortex biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
frontal cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: wild type
gender: male
age: 8 weeks
tissue: frontal cortex
|
| Treatment protocol |
Mice were orderd at 7 weeks of age and singly housed for 1 week. They were sacrificed by cervical dislocation at 9 am. Brains were quickly removed, rinsed with PBS, and placed on a filter paper on a petri dish filled with ice. Frontal cortex (cortical tissue dissected from a slice spanning Bregma -1.5 - -3.5) and the entire hippocampus were dissected and immediately frozen in liquid nitrogen, and stored at -80 °C until RNA extraction.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was homogenized using the Fastprep instrument and total RNA extracted using Trireagent
|
| Label |
biotin
|
| Label protocol |
FlashTag RNA Labeling with Genisphere RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip miRNA Array Procedure (FluidicsScript 450_0003, Hyb. 48°C)
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 (GCS3000), Affymetrix GeneChip Command Console (AGCC)
|
| Description |
miRNA expression data from C57BL/6J frontal cortex
|
| Data processing |
R/Bioconductor, RMA background correction, quintile normalization and summarization of the log-transformed expression values for mmu-miRNAs with affy package
|
| |
|
| Submission date |
Mar 10, 2011 |
| Last update date |
Jun 22, 2011 |
| Contact name |
Iiris Hovatta |
| E-mail(s) |
iiris.hovatta@helsinki.fi
|
| Organization name |
University of Helsinki
|
| Department |
Molecular Neurology
|
| Street address |
Haartmaninkatu 8
|
| City |
University of Helsinki |
| ZIP/Postal code |
00014 |
| Country |
Finland |
| |
|
| Platform ID |
GPL8786 |
| Series (1) |
| GSE27891 |
miRNA expression profiles of frontal cortex and hippocampus from adult C57BL/6J mice |
|
