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Sample GSM689066 Query DataSets for GSM689066
Status Public on Dec 14, 2011
Title IPSC 96 Cl 1
Sample type RNA
 
Source name Reprogrammed fibroblast into iPSC
Organism Homo sapiens
Characteristics cell line: iPSC 96
cell type: induced pluripotent stem cell
Growth protocol Human iPSC line derived from a 96 year old donor proliferative fibroblasts
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNeasy mini kits (Qiagen) according to the manufacturer's instructions and quantified using a NanoDrop spectrophotometer.
Label Biotin
Label protocol Complementary RNA (cRNA) was prepared according to the manufacturer’s (Affymetrix) protocol ‘small sample protocol II’, starting from 100 ng total RNA.

 
Hybridization protocol Following fragmentation, cRNA was hybridized for 16 hr at 45C on the GeneChip Human U133 plus 2.0 array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Data processing Data were analyzed with the GCOS 1.2 software (Affymetrix), using the default analysis settings and global scaling as normalization method, with a trimmed mean target intensity value (TGT) of each array arbitrarily set to 100.
 
Submission date Mar 11, 2011
Last update date Dec 14, 2011
Contact name Jean-Marc Lemaitre
E-mail(s) Jean-Marc.Lemaitre@igf.cnrs.fr
Organization name INSERM
Street address 141 rue de la cardonille
City Montpellier
ZIP/Postal code 34094
Country France
 
Platform ID GPL570
Series (1)
GSE27924 Microarray data for rejuvenation experiments through reprogramming

Data table header descriptions
ID_REF
VALUE signal intensity
ABS_CALL presence call
DETECTION P-VALUE p-value

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 249.189 P 9.4506e-05
AFFX-BioB-M_at 376.817 P 4.42873e-05
AFFX-BioB-3_at 240.337 P 4.42873e-05
AFFX-BioC-5_at 720.525 P 5.16732e-05
AFFX-BioC-3_at 856.23 P 4.42873e-05
AFFX-BioDn-5_at 1795.4 P 4.42873e-05
AFFX-BioDn-3_at 2725.51 P 4.42873e-05
AFFX-CreX-5_at 10065.6 P 5.16732e-05
AFFX-CreX-3_at 9894.84 P 4.42873e-05
AFFX-DapX-5_at 110.873 P 9.4506e-05
AFFX-DapX-M_at 249.238 P 0.000753643
AFFX-DapX-3_at 399.735 P 4.42873e-05
AFFX-LysX-5_at 19.1836 P 0.000340305
AFFX-LysX-M_at 20.6465 P 0.00844047
AFFX-LysX-3_at 58.4681 P 0.00010954
AFFX-PheX-5_at 30.3051 P 9.4506e-05
AFFX-PheX-M_at 35.1676 P 0.000753643
AFFX-PheX-3_at 42.1377 P 9.4506e-05
AFFX-ThrX-5_at 15.3302 P 0.0182806
AFFX-ThrX-M_at 44.2127 P 0.00110197

Total number of rows: 54675

Table truncated, full table size 1646 Kbytes.




Supplementary file Size Download File type/resource
GSM689066.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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