|
| Status |
Public on Jan 12, 2024 |
| Title |
HiC_Svb_Act_1 |
| Sample type |
SRA |
| |
|
| Source name |
Late embryonic derived S2 cells
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: Late embryonic derived S2 cells
cell type: S2 (Schneider 2) cell line
treatment: Expression of Svb-ACT
|
| Treatment protocol |
S2 cells were stably transfected with the repressive or the activated isoform of Shavenbaby (Svb) to study the influence of theproteasone mediated cleavage of this transcription factor. Exponentially growing cells were crosslinked and harvested as previously (Liang, Lacroix et al., Molecular Cell, 2014, Vol 53, 672-681).
|
| Growth protocol |
Schneider 2 (S2) Drosophila cells were cultured in Schneider Drosophila medium complemented with 10% heat-inactivated FBS and 1% pen-strep
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ARIMA HiC Kit
KAPA hyperprep
|
| |
|
| Library strategy |
Hi-C |
| Library source |
genomic |
| Library selection |
other |
| Instrument model |
DNBSEQ-T7 |
| |
|
| Data processing |
Raw data from different lanes (L2+L3) were merged before alignment.
alignment: Juicer version 1.6; BWA 0.7.17-r1188; openjdk version 1.8.0_302
Assembly: genome_version : dm6-r6.32
Supplementary files format and content: HiC contact matrix (.hic format)
|
| |
|
| Submission date |
Dec 28, 2022 |
| Last update date |
Jan 12, 2024 |
| Contact name |
Cédric Polesello |
| Organization name |
Centre de Biologie Intégrative
|
| Street address |
118 Route de Narbonne
|
| City |
Toulouse |
| ZIP/Postal code |
31062 |
| Country |
France |
| |
|
| Platform ID |
GPL32518 |
| Series (1) |
| GSE221863 |
Crosstalk between chromatin and the transcription factor Svb |
|
| Relations |
| BioSample |
SAMN32474057 |
| SRA |
SRX18866423 |
