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| Status |
Public on Jan 03, 2023 |
| Title |
Ctrl rep2 |
| Sample type |
SRA |
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| Source name |
Raw264.7
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| Organism |
Mus musculus |
| Characteristics |
cell line: Raw264.7
cell type: macrophage cell line
treatment: none
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| Treatment protocol |
AG group was induced by treatment of 1,5-Anhydroglucitol (0.5mM,J&K Scientific,China) for 24h.
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| Growth protocol |
Raw264.7 cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and antibiotics.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using using Trizol reagent following the manufacturer's protocol.
The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Raw reads were processed using Trimmomatic. The reads containing ploy-N and the low quality reads were removed to obtain the clean reads.
The clean reads were mapped to reference genome using hisat 2.
FPKM value of each gene was calculated using cufflinks, and the read counts of each gene were obtained by htseq-count.
Assembly: mm10
Supplementary files format and content: fpkm.csv
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| Submission date |
Dec 29, 2022 |
| Last update date |
Jan 03, 2023 |
| Contact name |
Min Wu |
| E-mail(s) |
mmw1997@126.com
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| Organization name |
Shandong University
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| Street address |
Wenhua West Road
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| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250000 |
| Country |
China |
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| Platform ID |
GPL24247 |
| Series (1) |
| GSE221889 |
Sequencing of Raw 264.7 macrophage cells treated with 1,5-Anhydroglucitol(1,5-AG). |
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| Relations |
| BioSample |
SAMN32489934 |
| SRA |
SRX18882008 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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