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Sample GSM691148 Query DataSets for GSM691148
Status Public on May 31, 2011
Title adipose_tissue_DM (16470)
Sample type RNA
 
Source name subcutaneous adipose tissue
Organism Homo sapiens
Characteristics clinical status: DM
bmi: 25.1
age: 62
fasting insulin: 42
fasting glucose: 7.7
hba1c: 6.4
vo2max ffm: 53
Treatment protocol Adipose tissue biopsies were obtained from the subcutaneous abdominal region (from the area below the umbilicus in a 4-cm range on either side) using the percutaneous needle biopsy technique with suction. Biopsies were quickly dissected free from visible blood and connective tissue and frozen in liquid nitrogen. To avoid interference of circadian rhythms and post-prandial responses, biopsies were obtained between 8 am and 10 am following an overnight fast.
Extracted molecule total RNA
Extraction protocol Frozen tissue was homogenised in Trizol (Invitrogen) using a motor-driven homogenizer (Polytron, Kinematica, Newark, NY, USA). RNA was extracted according to manufacturer’s protocol.
Label biotin
Label protocol As per Affymetrix directions
 
Hybridization protocol As per Affymetrix directions
Scan protocol As per Affymetrix directions
Data processing All array data were normalized using the Microarray Suite version 5.0 (MAS 5.0) algorithm to a global scaling intensity of 100. Data were filtered using present/absent calls. Probes called absent by MAS 5.0 across all arrays were discarded. Further data processing were performed by RMA.
 
Submission date Mar 14, 2011
Last update date May 31, 2011
Contact name Iain Gallagher
E-mail(s) iaingallagher@gmail.com
Phone 00 44 1786 46 6024
Organization name University of Stirling
Department Faculty of Health Sciences and Sport
Street address Room 4B133 Cottrell Building, University of Stirling, Airthrey Road
City Stirling
ZIP/Postal code FK9 4LA
Country United Kingdom
 
Platform ID GPL570
Series (2)
GSE27949 Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity (Affymetrix)
GSE27951 Gene-chip studies of adipogenesis-regulated microRNAs in mouse primary adipocytes and human obesity

Data table header descriptions
ID_REF
VALUE RMA normalised data.

Data table
ID_REF VALUE
1007_s_at 7.925824875
1053_at 5.334950057
117_at 5.484470629
121_at 6.689023431
1255_g_at 2.719723403
1294_at 7.773337662
1316_at 5.145862983
1320_at 3.894160937
1405_i_at 5.73370221
1431_at 3.145581673
1438_at 4.49911593
1487_at 6.744181832
1494_f_at 5.050095099
1552256_a_at 8.782040674
1552257_a_at 6.170014836
1552258_at 3.88087328
1552261_at 4.407138973
1552263_at 4.368657333
1552264_a_at 5.325935174
1552266_at 3.584925286

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM691148.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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