|
Status |
Public on Jan 01, 2024 |
Title |
Corona radiata WT 1 |
Sample type |
SRA |
|
|
Source name |
Cumulus WT
|
Organism |
Mus musculus |
Characteristics |
genotype: WT tissue: Corona radiata - granulosa age: Adult
|
Treatment protocol |
Granulosa cell samples post J4 PMSG/hCG induction
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA were extracted with Nacherey-Nagel RNA L extraction kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
WT1
|
Data processing |
Quality score trimming and filtering were performed with Cutadapt. Trimmed reads were aligned to the annotated mouse genome (mm10) using Hisat2 (v2.1.0) Per gene read counts were calculated from the Hisat2 output using htseq-count (v0.5.4p5) RPKM (Reads Per Kilobase of exon per Megabase of library size) were caclulated Differential gene expression was analyzed using the R package DESeq2 (v1.2.8) Assembly: mm10 Supplementary files format and content: tab-delimited text files include ENS id gene symbol and RPKM values for each sample.
|
|
|
Submission date |
Jan 04, 2023 |
Last update date |
Jan 01, 2024 |
Contact name |
yoan renaud |
E-mail(s) |
yoan.renaud@uca.fr
|
Organization name |
igred
|
Street address |
place henri dunand
|
City |
clermont ferrand |
ZIP/Postal code |
63000 |
Country |
France |
|
|
Platform ID |
GPL19057 |
Series (1) |
GSE222134 |
Transcriptomic analysis of corona radiata cells after hormonal stimulation (PMSG/hCG) |
|
Relations |
BioSample |
SAMN32575789 |
SRA |
SRX18924227 |