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Sample GSM6915506 Query DataSets for GSM6915506
Status Public on Jan 01, 2024
Title Corona radiata WT 1
Sample type SRA
 
Source name Cumulus WT
Organism Mus musculus
Characteristics genotype: WT
tissue: Corona radiata - granulosa
age: Adult
Treatment protocol Granulosa cell samples post J4 PMSG/hCG induction
Extracted molecule total RNA
Extraction protocol RNA were extracted with Nacherey-Nagel RNA L extraction kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description WT1
Data processing Quality score trimming and filtering were performed with Cutadapt.
Trimmed reads were aligned to the annotated mouse genome (mm10) using Hisat2 (v2.1.0)
Per gene read counts were calculated from the Hisat2 output using htseq-count (v0.5.4p5)
RPKM (Reads Per Kilobase of exon per Megabase of library size) were caclulated
Differential gene expression was analyzed using the R package DESeq2 (v1.2.8)
Assembly: mm10
Supplementary files format and content: tab-delimited text files include ENS id gene symbol and RPKM values for each sample.
 
Submission date Jan 04, 2023
Last update date Jan 01, 2024
Contact name yoan renaud
E-mail(s) yoan.renaud@uca.fr
Organization name igred
Street address place henri dunand
City clermont ferrand
ZIP/Postal code 63000
Country France
 
Platform ID GPL19057
Series (1)
GSE222134 Transcriptomic analysis of corona radiata cells after hormonal stimulation (PMSG/hCG)
Relations
BioSample SAMN32575789
SRA SRX18924227

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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