|
| Status |
Public on Mar 15, 2016 |
| Title |
MDAMB231_siLUC_ctrl |
| Sample type |
RNA |
| |
|
| Source name |
MDAMB231 breast cancer cells, siRNA pool, knockdown luciferase
|
| Organism |
Homo sapiens |
| Characteristics |
organism: Homo sapiens
cell line: MDA-MB231 (ATCC)
cell type: breast carcinoma cells
knockdown: transient
|
| Biomaterial provider |
Dr. Bram De Craene, Molecular and Cellular Oncology, VIB & Ghent University, Ghent Belgium
|
| Treatment protocol |
MDAMB231 cells were transfected with 20 nM siRNA using the Dharmafect transfection reagent following the manufacturer's instructions (Dharmacon). Twenty-four hours later, the cells were extensively washed with PBS, incubated for 48 hours in culture medium and then total RNA was harvested.
|
| Growth protocol |
The human MDAMB231 breast carcinoma cell line was obtained from the American Type Tissue Collection. Cells were maintained in Leibovitz-15 with 10% FCS, 200 nM L-glutamine, 100 µg/ml penicillin, and 100 µg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Mini kit for total RNA purification according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45°C and at 60 rounds/min. The GeneChips were washed and stained in the Affymetrix Fluidics Station 400 and 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Data processing |
RMA-normalized expression values as obtained with the affy package of BioConductor. The reported values are on log2-scale.
|
| |
|
| Submission date |
Mar 15, 2011 |
| Last update date |
Mar 15, 2016 |
| Contact name |
Rekin's Janky |
| E-mail(s) |
Nucleomics.Bioinformatics@vib.be
|
| Organization name |
VIB
|
| Department |
Nucleomics Core
|
| Street address |
Herestraat 49 Box 816
|
| City |
Leuven |
| ZIP/Postal code |
B-3000 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27966 |
Loss of ZEB2 in basal breast cancer cells |
|
