|
| Status |
Public on Apr 30, 2024 |
| Title |
splenic sorted NK cells, wild type, d2 MCMV, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
sorted splenic NK cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: sorted splenic NK cells
cell type: NK cells (Ly49H+)
genotype: WT
time: D2
treatment: NK cell enrichment followed by FACS sort for Ly49H+ NK1.1+ CD3-
|
| Extracted molecule |
total RNA |
| Extraction protocol |
bulk RNA was isolated from sorted, splenic NK1.1+ CD3/TCRβ- Ly49H+ cells using the Nucleospin RNA isolation kit
MGIEasy V3 chemistry
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
MGISEQ-2000RS |
| |
|
| Description |
column 9
|
| Data processing |
Raw paired end fastq files were aligned to the UCSC mm10 genome with RNAStar, followed by featureCounts and DESeq2 (standard parameters)
Assembly: UCSC mm10
Supplementary files format and content: tab-delimited text files include CPM values for each sample
|
| |
|
| Submission date |
Jan 05, 2023 |
| Last update date |
Apr 30, 2024 |
| Contact name |
Sebastian Scheer |
| E-mail(s) |
sebastian.scheer@lih.lu
|
| Organization name |
Luxembourg Institute of Health
|
| Department |
Infection and Immunity
|
| Lab |
Endocrine and Epigenetics
|
| Street address |
29, rue Henri Koch
|
| City |
Esch-sur-Alzette |
| ZIP/Postal code |
4354 |
| Country |
Luxembourg |
| |
|
| Platform ID |
GPL30215 |
| Series (2) |
| GSE222233 |
DOT1L controls NK cell maturation and lineage integrity [RNA-seq D0 & D2] |
| GSE222235 |
DOT1L controls NK cell maturation and lineage integrity |
|
| Relations |
| BioSample |
SAMN32601103 |
| SRA |
SRX18944361 |
