|
| Status |
Public on Mar 16, 2011 |
| Title |
HL-1 cardiomyocyte hypox2 |
| Sample type |
RNA |
| |
|
| Source name |
HL-1 cardiomyocyte
|
| Organism |
Mus musculus |
| Characteristics |
cell line: HL-1
culture: Hypoxia (1% O2)
|
| Treatment protocol |
Confluent cells were incubated in hypoxia (1% O2) or normoxia (21% O2) for 8 hours before harvest.
|
| Growth protocol |
HL-1 cells were grown according to standard protocol, as described in Claycomb WC et. al. Proc Natl Acad Sci U S A 95: 2979-2984, 1998.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
QIAGEN Rneasy Mini Kit, extraction according to protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation,15 ug of biotinylated cRNA were hybridized for 16 hr at 45C MOE430A microarrays according to the standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol (G2500A GeneArray scanner) Standrd Affymetrix protocol, (
|
| Description |
HL-1 cardiomyocyte hypox2
|
| Data processing |
MAS 5, signal algorithm, global scaling (target intensity 100)
|
| |
|
| Submission date |
Mar 15, 2011 |
| Last update date |
Mar 16, 2011 |
| Contact name |
Jeanna C Perman |
| E-mail(s) |
jeanna.perman@wlab.gu.se
|
| Organization name |
Wallenberg Laboratory for cardiovascular research
|
| Street address |
Bruna Stråket 16
|
| City |
Gothenburg |
| ZIP/Postal code |
41345 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27975 |
HL-1 cardiomyocyte response to hypoxia |
|
