Cells were grown in RPMI-1640 medium supplemented with 10% heat-inactivated fetal calf serum (FCS), 2 mmol/ml L-glutamine, 0.01 M HEPES buffered saline, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 12.5 units/ml nystatin. All cells were routinely cultured in humidified air with 5% CO2 at 37°C. The cells were routinely tested for the presence of mycoplasma.
Extracted molecule
total RNA
Extraction protocol
total RNA was isolated using the Qiagen Rneasy Mini Kit (Qiagen, Valencia, CA). Samples were treated for DNase using DNase Set (Qiagen). RNA concentrations were determined by the absorbance at 260 nm and quality control standards were A260/A280 = 1.8 – 2.0.
Label
biotin
Label protocol
according to the instruction manual, as described in the Affymetrix website
Hybridization protocol
according to the instruction manual, as described in the Affymetrix website
Scan protocol
according to the instruction manual, as described in the Affymetrix website
Description
RKTJ-first cycle of intrcardiac inoculation, brain metastasis variant, replicate 1 Gene expression of RKTJ, brain metastasis variant
