|
| Status |
Public on Apr 03, 2023 |
| Title |
CCl4-induced liver fibrosis for 10 weeks, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Fibrosed liver, 10 weeks of CCl4 injections, biological replicate no 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Liver
gentoype: C57Bl/6N
age: 20 w
|
| Treatment protocol |
Mice were subjected to 1.6 g/kg CCl4 i.p. twice/week. Then two days after last CCl4 injection mice were sacrificed and liver was harvested
|
| Growth protocol |
CCl4-induced fibrosis is described in https://doi.org/10.21203/rs.3.rs-2009380/v1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total liver RNA isolation was performed using InviTrap®Spin Universal RNA Mini Kit from Stratec (1060100300, Birkenfeld, Germany) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression of mouse fibrose liver 10 weeks after CCl4 induction
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 8, from SAS. Gene expression profiling was performed using arrays of Mogene-2_0 type from Affymetrix. A Custom CDF Version 20 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Jan 10, 2023 |
| Last update date |
Apr 03, 2023 |
| Contact name |
Carolina Delatorre |
| E-mail(s) |
carolina.delatorre@medma.uni-heidelberg.de
|
| Organization name |
University Heidelberg
|
| Street address |
Theodor-Kutzer-Ufer 1-3
|
| City |
Mannheim |
| ZIP/Postal code |
68167 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22598 |
| Series (1) |
| GSE222576 |
Transcriptional Profiling of liver fibrosis induced by CCl4 injections |
|
