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| Status |
Public on Jan 15, 2024 |
| Title |
Male Aedes albopictus-2 |
| Sample type |
SRA |
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| Source name |
whole body
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| Organism |
Aedes albopictus |
| Characteristics |
tissue: whole body
strain: Foshan
Sex: Male
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| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted with Takara RNAiso Plus (Takara, Dalian, China)
circRNA-seq library was prepared with approximately 1μg of total RNA using KAPA RNA HyperPrep Kit with RiboErase (HMR) for Illumina® (Kapa Biosystems, Inc., Woburn, MA) and using RNase R (geneseed, Inc., Guangzhou, China) to digest linear RNA . Briefly, ribosomal RNA was removed from the total RNA. Next, the ribominus RNAs were fragmented and then first strand and directional second strand synthesis were performed. Then the A tailing and adapter ligation were performed with the purified cDNA. Finally, the purified, adapter-ligated DNA was amplified. The library quality and concentration was assessed by utilizing a DNA 1000 chip on an Agilent 2100 Bioanalyzer.
HiSeq X10 PE150
circRNA-seq
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
HiSeq X Ten |
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| Description |
TMM_normalized_CPM.txt
circRNA_reads_count.txt
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| Data processing |
TMM (trimmed mean of M-values) was used to normalize the circRNA expression
Sequenced reads were trimmed for adapter sequence, and masked for low-complexity or low-quality sequence, then mapped to Aedes albopictus whole genome using Bowtie2 v2.1.0
Assembly: Aedes albopictus
Supplementary files format and content: tab-delimited txt file includes TMM values for each Sample
Supplementary files format and content: Matrix table with circRNA reads count for every circRNA and every sample
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| Submission date |
Jan 11, 2023 |
| Last update date |
Jan 15, 2024 |
| Contact name |
Jinbao Gu |
| E-mail(s) |
daipeng217@126.com
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| Organization name |
Southern Medical University
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| Department |
Department of Pathogen Biology, School of Public Health
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| Street address |
No. 1023, Shatai South Road
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| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510515 |
| Country |
China |
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| Platform ID |
GPL33013 |
| Series (1) |
| GSE222630 |
Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis |
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| Relations |
| BioSample |
SAMN32676259 |
| SRA |
SRX19002651 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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