|
| Status |
Public on Jan 12, 2023 |
| Title |
Subject SU009 |
| Sample type |
protein |
| |
|
| Source name |
Serum/Plasma
|
| Organism |
Homo sapiens |
| Characteristics |
condition: ICU Patient from Stanford
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
protein |
| Extraction protocol |
Blood was collected from patients and healthy controls. The blood was then processed into serum or plasma which was then run on our arrays.
|
| Label |
R-PE conjugated goat anti-human IgG secondary antibody
|
| Label protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
| |
|
| Hybridization protocol |
A secondary antibody conjugated to a fluorophore was added to a PBS solution containing conjugated beads bound to antibodies from samples.
|
| Scan protocol |
A Luminex FlexMAP 3D instrument was used to measure fluorescence (in MFI) from each barcoded bead.
|
| Description |
Antibodies in samples bind to conjugated beads in array. The fluorescence measured as MFI from the secondary antibody was used to determine antibody reactivity.
|
| Data processing |
Sample replicate MFI values were averaged for each antigen. The average barebead MFI was then subtracted from the average MFI of each sample and antigen.
|
| |
|
| Submission date |
Jan 12, 2023 |
| Last update date |
Jan 12, 2023 |
| Contact name |
Allan Feng |
| E-mail(s) |
afeng1@stanford.edu
|
| Phone |
2055678956
|
| Organization name |
Stanford School of Medicine
|
| Department |
Imm/Rheum
|
| Lab |
Utz
|
| Street address |
269 Campus Dr
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL33018 |
| Series (2) |
| GSE222764 |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness [CTD] |
| GSE222765 |
Autoantibodies are highly prevalent in non-SARS-CoV-2 respiratory infections and critical illness |
|
