|
| Status |
Public on Jan 26, 2023 |
| Title |
S2 cells, Control KD, OE WT, replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
S2 cell line
|
| Organism |
Drosophila melanogaster |
| Characteristics |
cell line: S2 cell line
cell type: late embryonic stage
genotype: Luc knockdown
treatment: transfection Ac-Da
|
| Treatment protocol |
Description in the Materials and Methods section of paper.
|
| Growth protocol |
Drosophila S2 cells were maintained in Schneider's Drosophila medium supplemented with 10% fetal bovine serum (FBS) at 25C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol (Invitrogen). 1.0 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using MGIEasy RNA Library Prep Kit or TruSeq strandard mRNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
DNBSEQ-G400 |
| |
|
| Description |
Luc ctrl KD_WT, replicate 2
|
| Data processing |
Partek® Flow® Genomic Analysis Software
Sequence reads were trimmed for adaptor sequence/low-quality sequence using Partek® Flow® Genomic Analysis Software
Trimmed sequence reads were mapped to BDGP6.32 using Partek® Flow® Genomic Analysis Software
Read count extraction and normalization were performed using Partek® Flow® Genomic Analysis Software
Assembly: BDGP6.32
Supplementary files format and content: tab-delimited text files include raw read counts for each Sample
|
| |
|
| Submission date |
Jan 21, 2023 |
| Last update date |
Jan 26, 2023 |
| Contact name |
Yun-Ling Hsiao |
| E-mail(s) |
yunlingh@gmail.com
|
| Organization name |
Chang Gung University
|
| Street address |
No. 259, Wenhua 1st Road
|
| City |
Taoyuan City |
| State/province |
Guishan Dist. |
| ZIP/Postal code |
33302 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL32218 |
| Series (1) |
| GSE223416 |
Actin-related protein 6 facilitates proneural protein-induced gene activation for rapid neural differentiation |
|
| Relations |
| BioSample |
SAMN32850700 |
| SRA |
SRX19127005 |
