|
| Status |
Public on Jan 28, 2023 |
| Title |
4- MEF Tfam+/-_rep2 |
| Sample type |
SRA |
| |
|
| Source name |
mouse embryo
|
| Organism |
Mus musculus |
| Characteristics |
tissue: mouse embryo
cell type: primary mouse embryonic fibroblasts
genotype: Tfam+/-
treatment: none
|
| Treatment protocol |
none
|
| Growth protocol |
MEFs were grown in DMEM media (Sigma D5796) supplemented with 10% FBS (screened for low endotoxin; VWR 97068-085). After two passages, cells were harvested for RNA extraction. Standard culture at 37C, 5% CO2 in a humidified incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was prepared using the Quick-RNA microprep kit (Zymo Research).
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina BaseSpace FASTQ Generation | Version: 1.0.0
Illumina BaseSpace RNA-Seq Alignment | Version: 2.0.0; Incroporates STAR Aligner (Version: 2.6.1a) and Salmon Quantification (Version 0.11.2)
Assembly: Mus_musculus (UCSC mm10)
Supplementary files format and content: MEF RNA_seq normalized counts.txt
|
| |
|
| Submission date |
Jan 23, 2023 |
| Last update date |
Jan 28, 2023 |
| Contact name |
Phillip West |
| E-mail(s) |
awest@tamu.edu
|
| Organization name |
Texas A&M University School of Medicine
|
| Department |
Microbial Pathogenesis and Immunology
|
| Street address |
8447 Riverside Parkway, MREB 1 3012
|
| City |
Bryan |
| State/province |
TX |
| ZIP/Postal code |
77807 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE223553 |
Transcriptomic analysis of WT, Tfam+/-, Zbp1-/- and Tfam+/-Zbp1-/- mouse embryonic fibroblasts (MEFs) |
|
| Relations |
| BioSample |
SAMN32881939 |
| SRA |
SRX19145020 |
