|
Status |
Public on Sep 05, 2012 |
Title |
H0033176 |
Sample type |
RNA |
|
|
Source name |
T1284C
|
Organism |
Homo sapiens |
Characteristics |
tissue: cancerous tissue
|
Treatment protocol |
Tissues were snap-frozen and stored at -80°C until further use.
|
Extracted molecule |
total RNA |
Extraction protocol |
Human tissues measured approximately 60mm3 in volume were homogenized using the PowerGen 125 (Fisher Scientific, Pittsburgh, PA) in 600 µL of Lysis/Binding buffer provided in the mirVanaTM miRNA Isolation kit (Ambion/Applied Biosystems, Austin, TX) and then followed the manufacturer’s instruction to isolate total RNA.
|
Label |
Cy3
|
Label protocol |
100 ng of total RNA per sample was dephosphorylated with calf intestine alkaline phosphatase and the pCp-Cy3 labeling molecule was ligated to the 3ʹ-ends of the RNA molecules, and then was purified using BioSpin6 (Bio-Rad, Hercules, CA).
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|
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Hybridization protocol |
Hybridization, washing, imaging, and signal extraction were performed according to Agilent-recommended procedures.
|
Scan protocol |
Slides were scanned on Agilent DNA microarray scanner (model G2565A).
|
Description |
MicroRNA expression in oral squamous cell carcinoma tissue
|
Data processing |
The scanned signals were extracted using Agilent Feature Extraction (FE) software version 9.5.3.1 to perform background correction, outlier rejection for each set of replicate features, and calculation of total gene signal for each probe on the array.
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|
|
Submission date |
Mar 22, 2011 |
Last update date |
Sep 05, 2012 |
Contact name |
Hyun Min Jung |
E-mail(s) |
hyunmin.jung@nih.gov
|
Phone |
301-496-7940
|
Organization name |
National Institutes of Health
|
Department |
Division of Developmental Biology
|
Lab |
Weinstein Lab
|
Street address |
6 Center Drive
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL10850 |
Series (1) |
GSE28100 |
Differential expression of microRNAs between oral squamous cell carcinoma and healthy control tongues |
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