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| Status |
Public on Nov 01, 2011 |
| Title |
NMuMG_Ex_TGF-beta |
| Sample type |
RNA |
| |
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| Source name |
normal murine mammary gland epithelial cell line (NMMuMG), treated with TGF-beta
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| Organism |
Mus musculus |
| Characteristics |
cell line: NMuMG
tissue origin: mammary gland
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| Treatment protocol |
Cells were treated with 1 ng/ml recombinant human TGF-beta1 (R&D Systems, Minneapolis, MN) or left untreated.
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| Growth protocol |
Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen, Carlsbad, CA) supplemented with 4.5 g/l glucose, 10% fetal bovine serum (FBS), 10 ug/ml insulin, 50 U/ml penicillin, and 50 ug/ml streptomycin.
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| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified using the RNeasy Mini Kit (Qiagen, Valencia, CA).
|
| Label |
biotin
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| Label protocol |
Labeling was performed according to the protocols provided by Affymetrix.
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| Hybridization protocol |
Hybridization was performed according to the protocols provided by Affymetrix, using GeneChip Hybridization, Wash, and Stain Kit (Affymetrix).
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| Scan protocol |
Scanning was performed according to the protocols provided by Affymetrix.
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| Description |
exon level data
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| Data processing |
CEL files were generated using Affymetrix GeneChip Command Console Software (AGCC).
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| Submission date |
Mar 25, 2011 |
| Last update date |
Nov 01, 2011 |
| Contact name |
Daizo Koinuma |
| E-mail(s) |
d-koinuma@umin.ac.jp
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| Organization name |
University of Tokyo
|
| Department |
Pathology
|
| Street address |
Hongo 7-3-1, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
| |
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| Platform ID |
GPL6193 |
| Series (1) |
| GSE28184 |
Expression data from normal murine mammary epithelial cell line NMuMG stimulated with TGF-beta |
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| Relations |
| Alternative to |
GSM697691 (gene-level analysis) |
| Data table header descriptions |
| ID_REF |
|
| VALUE |
RMA singal estimates of CORE probesets from AGCC. |
| Data table |
| ID_REF |
VALUE |
| 4304927 |
39.55107 |
| 4304928 |
3664.24 |
| 4304938 |
683.3591 |
| 4304943 |
57.50614 |
| 4304945 |
1675.498 |
| 4304949 |
584.9769 |
| 4304956 |
144.5399 |
| 4304969 |
5426.533 |
| 4304972 |
257.6943 |
| 4304978 |
283.1111 |
| 4304981 |
149.5168 |
| 4304986 |
65.41071 |
| 4304997 |
285.5071 |
| 4305011 |
24.72499 |
| 4305016 |
79.77313 |
| 4305019 |
48.19714 |
| 4305028 |
1142.97 |
| 4305034 |
11782.02 |
| 4305036 |
22.01916 |
| 4305039 |
74.78574 |
Total number of rows: 229581
Table truncated, full table size 3786 Kbytes.
| Supplementary file |
Size |
Download |
File type/resource |
| GSM697688_02_NMuMG_Tb_24h-_No_1082_MoEx-1_0-st-v1_.CEL.gz |
24.3 Mb |
(ftp)(http) |
CEL |
| Processed data included within Sample table |
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