|
| Status |
Public on Sep 08, 2011 |
| Title |
SK mature embryos: 13˚C vs 25˚C repeat2 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
13S9
|
| Organism |
Triticum aestivum |
| Characteristics |
cultivar: Shiroganekomugi
developmental stage: embryo
temperature: 13˚C
daa: 80
|
| Treatment protocol |
After anthesis, the temperautures of the rooms were adjusted and maintained at 13˚C and 25˚C, repectively. The spikes were harvested at the indicated DAA.
|
| Growth protocol |
Wheat were grown in adjoining two rooms in a green house.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The yield and RNA purity were determined spectrophotometrically. Integrity was verified using an Agilent 2100 Bioanalyzer
|
| Label |
Cy3
|
| Label protocol |
TotalRNA (400 ng) was labeled with Cy3 or Cy5 using an Agilent Quick Amp Labeling kit .
|
| |
|
| Channel 2 |
| Source name |
25S10
|
| Organism |
Triticum aestivum |
| Characteristics |
cultivar: Shiroganekomugi
developmental stage: embryo
temperature: 25˚C
daa: 43
|
| Treatment protocol |
After anthesis, the temperautures of the rooms were adjusted and maintained at 13˚C and 25˚C, repectively. The spikes were harvested at the indicated DAA.
|
| Growth protocol |
Wheat were grown in adjoining two rooms in a green house.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using Trizol following manufacturer's instructions. The yield and RNA purity were determined spectrophotometrically. Integrity was verified using an Agilent 2100 Bioanalyzer
|
| Label |
Cy5
|
| Label protocol |
TotalRNA (400 ng) was labeled with Cy3 or Cy5 using an Agilent Quick Amp Labeling kit .
|
| |
|
| |
| Hybridization protocol |
The hybridization and washing were performed according to the Agilent manufacturer’s instructions
|
| Scan protocol |
Scanned on an Agilent MicroarrayScanner
Images were quantified using Agilent Feature Extraction Software (version 9.1).
|
| Description |
Biological Repeat
|
| Data processing |
Agilent Feature Extraction Software (v 9.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Sep 08, 2011 |
| Contact name |
Shingo Nakamura |
| E-mail(s) |
shingo@affrc.go.jp
|
| Organization name |
National Institute of Crop Science
|
| Lab |
Wheat and Barley Biotechnology Research Lab
|
| Street address |
Kannondai 2-1-18
|
| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8518 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9805 |
| Series (1) |
| GSE22786 |
Embryos of wheat seeds: Temperature effect |
|
