|
| Status |
Public on Dec 30, 2011 |
| Title |
Rab25 IGF treated 2 |
| Sample type |
RNA |
| |
|
| Source name |
Rab25, 24h IGF expt 2
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: A2780
|
| Treatment protocol |
Cells were serum straved for 8h before addition of growth factor EGF (10ng/ml) , IGF (10ng/ml), TGF beta (5ng/ml) and FBS (5%) for 8h (expt 1) or 24h (expt 2)
|
| Growth protocol |
pcDNA or Rab25 transfected cells were maintained in 5% RPMI 1640
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were collected after two-washings with PBS. The cell pellets were immediately frozen in liquid nitrogen, and stored until use. Total RNA was extracted using QIAGEN RNeasy mini kit (QIAGEN, Inc., Valencia, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 48C on Affy. GeneChips were washed and stained on the Affymetrix High-throughput array platform
|
| Scan protocol |
GeneChips were scanned using the Affymetrix High Throughput (HT) scanner. The software program then converts these .dat files into mini .cel files and then into composite cel files where the information is analyzed in the Affymetrix GCOS 1.2 software.
|
| Description |
A2780 Rab25 I2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. RMA with default parameters was used to create a final gene expression data. We used BRB array Tool V.3 to create the RMA and log 2 value.
|
| |
|
| Submission date |
Mar 31, 2011 |
| Last update date |
Dec 30, 2011 |
| Contact name |
Kwai Wa Cheng |
| Organization name |
UT-MD Anderson Cancer Center
|
| Department |
Systems Biology
|
| Street address |
1515 Holcombe Blvd.
|
| City |
Houston |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL4685 |
| Series (1) |
| GSE28299 |
Transcription profiling of Rab25 transfected ovarian A2780 cancer cell |
|
